‘巴斗杏’组培快繁体系建立与耐盐植株筛选  

Establishment of Tissue Culture and Rapid Propagation System of Armeniaca vulgaris 'Badou'and Selection of Salt-tolerant Plant

作　　者：杜雪玲[1] 余如刚[1] 宋运贤[1] 张振霞[2] 

机构地区：[1]淮北师范大学生命科学学院资源植物学安徽省重点实验室,安徽淮北235000 [2]韩山师范学院生物系,广东潮州521041

出　　处：《亚热带植物科学》2011年第2期34-38,共5页Subtropical Plant Science

基　　金：安徽省高校自然科学研究项目(KJ2008B230)

摘　　要：以淮北黄里‘巴斗杏’茎段为外植体,MS为基本培养基,通过茎段诱导植株再生及进行耐盐筛选。结果表明:采用4、5月‘巴斗杏’茎段用0.1%升汞灭菌8 min较适宜;在含有1.0 mg/L 6-BA和0.1 mg/L IBA的MS培养基上茎段增殖较快,生长旺盛;较理想的生根培养基为MS+NAA 0.1 mg/L+IBA 0.2 mg/L,生根率达46.3%;‘巴斗杏’组培苗进行耐盐筛选的适宜盐浓度为0.4%～0.8%,筛选植株与对照相比差异显著。The stem ofArmeniaca vulgaris ＇Badou＇ was used as explants and MS as basic substrate to induce plant regeneration and select salt-tolerant plant. The results showed that the stem of ＇Badou＇ in April and May sterilized by 0.1% mercury for 8min was suitable, the stem in MS culture medium with 1.0mg/L 6-BA and 0.1 mg/L IBA proliferated quickly and grew rushingly, the perfect rooting medium was MS＋0.1 mg/L NAA ＋ 0.2 mg/L IBA, with its rooting rate of 46.3%, and the optimum salt concentration in selection of salt-tolerant tissue culture seedlings was 0.4-0.8, there were also significant difference between plants selected and their contrast.

关 键 词：'巴斗杏’ 腋芽 增殖 生根 耐盐筛选 

分 类 号：Q943.1[生物学—植物学]