正交试验法确定烟草甲成虫羧酸酯酶反应的最适条件  

The Optimization of Conditions for Assaying Activity of Carboxylesterase from the Adults of Cigarette Beetle Lasioderma serricorne (Fabrcius) by Orthogonal Matrix Method

作　　者：柳琼友[1] 陈文龙[1] 顾丁[1] 高光澜[1] 

机构地区：[1]贵州大学昆虫研究所贵州省山地农业病虫害重点实验室,贵阳550025

出　　处：《西南师范大学学报（自然科学版）》2011年第3期150-155,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：中国烟草总公司贵州省公司科技资助项目(合同号200901);贵州大学引进人才科研资助项目[贵大人基合字(2007)031号]

摘　　要：应用正交表L25(56)设计正交试验,研究了羧酸酯酶质量浓度、体积分数浓度、反应体系的pH值、反应温度和反应时间等5个因素对烟草甲成虫羧酸酯酶活性检测的影响,并从试验组合中选出最佳条件.在进行极差分析、方差分析和多重比较后,选择各因素的最优水平,得到了烟草甲成虫羧酸酯酶活性测定的最适条件,即酶的质量浓度为1头/mL、底物的体积分数为5.0×10-4mol/L,pH值为7.5,温度为42℃,时间为10 min.The affects of CarE concentration,substrate a-Naphthyl acetate（a-NA） concentration,pH of the reaction system,the reaction temperature and the reaction time on assaying of the specific activity of CarE in the adults of Lasioderma serricorne（Fabrcius） were studied by orthogonal matrix methods.L25（56） orthogonal matrix was adopted without considering interaction.The optimal conditions for assaying the activity of CarE in the adult beetles,through range analysis,analysis of variance and multiple comparison,were considered as 1 beetle/mL,5.0×10-4 mol/L substrate,pH 7.5,42 ℃,and 10 minutes.

关 键 词：烟草甲 羧酸酯酶 正交试验 方差分析 最佳条件 

分 类 号：Q969.496.1[生物学—昆虫学]