淫羊藿总黄酮对糖尿病小鼠血管功能的影响  

Effects of Total Flavonoids of Epimedium on Vascular Function of Diabetic Mice

作　　者：韩爱萍[1] 张洁[1] 丁选胜[1] 

机构地区：[1]中国药科大学临床药学教研室,江苏南京210009

出　　处：《南京中医药大学学报》2011年第3期243-246,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81073145)

摘　　要：目的研究淫羊藿总黄酮(total flavonoids of Epimedium,TFE)对四氧嘧啶所致糖尿病小鼠离体胸主动脉舒缩功能的影响及其可能机制。方法雄性昆明种小鼠腹腔注射四氧嘧啶诱导糖尿病,对照组小鼠注射等体积的生理盐水。将成模小鼠按血糖值分为模型组、淫羊藿总黄酮低剂量组、淫羊藿总黄酮高剂量组。给药2周后,测空腹血糖(FBG)、超氧化物歧化酶(SOD)和丙二醛(MDA)。并观察各组小鼠胸主动脉对苯肾上腺素(phenylephrine,Phe)缩血管作用的反应,对乙酰胆碱(ACh)、硝普钠(SNP)舒血管作用的反应,同时体外加药观察药物的舒血管作用。结果 TFE能显著降低糖尿病小鼠FBG,使SOD活性升高、MDA含量降低,糖尿病小鼠胸主动脉的内皮依赖性舒张得到明显改善。结论淫羊藿总黄酮可能通过降低血糖、抗氧化等途径对糖尿病小鼠血管内皮损伤起到保护作用。OBJECTIVE To study the effects of total flavonoids of epimedium（TFE） on systolic and diastolic functions of thoracic aortic in diabetic mice and its possible mechanism.METHODS Kunming male mice were administered intraperitoneally with alloxan solution to induce type I diabetes and the control mice were injected with the same volume of saline.The diabetic mice were randomly divided into model group,low-dose TFE group,and high-dose TFE group according to the blood glucose.Serum fasting blood glucose（FBG）,superoxide dismutase（SOD） and malondialdehyde（MDA） were tested 2 weeks after continuous administration.The vasoconstriction response to phenylephrine（Phe） and the vasodilatation response to acetylcholine（ACh）,sodium nitroprusside（SNP） of isolated mice thoracic aorta were observed,also the vasodilatation effect of the TFE in vitro were observed in the normal group and model group.RESULTS After administrating TFE for two weeks,the level of FBG and MDA decreased,the level of SOD increased,and the endothelium-dependent relaxation of thoracic aortas significantly improved in the model group.CONCLUSION The TFE may have a protective effect on the vascular endothelial injury in diabetic mice through decreasing blood glucose and the anti-oxidative stress.

关 键 词：淫羊藿总黄酮 糖尿病小鼠 胸主动脉 血管功能 

分 类 号：R285.5[医药卫生—中药学]