滋补肾精方对肾精不足证治疗机制的实验研究  

Experimental Research on the Mechanism of Zibu Shenjing Fang in Treating Mice with Kidney Essence Deficiency

作　　者：苏小军[1] 王玉萍[1] 时吉萍[1] 王文辉[1] 张茂祥[1] 姚军孝[1] 何岳珍[1] 沈世林[1] 

机构地区：[1]兰州大学第一医院,甘肃兰州730000

出　　处：《南京中医药大学学报》2011年第3期257-259,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：甘肃省卫生行业科研计划项目

摘　　要：目的观察滋补肾精方对肾精不足证的治疗作用并探讨补肾填精的机理。方法将50只小鼠随机分为正常组、模型组、金匮肾气丸组以及滋补肾精方高剂量组、低剂量组。用恐伤肾联合劳倦过度建造肾精不足证动物模型。造模同时,金匮肾气丸组灌胃金匮肾气丸混悬液2.7 g/(kg.d),滋补肾精方高、低剂量组分别以滋补肾精方20、10 g/(kg.d)灌胃,连续21 d。观察小鼠一般状况,体质量、胸腺、脾脏指数及血清总抗氧化力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)活性。结果滋补肾精方能够改善小鼠肾精不足证表现,增加小鼠体质量,升高胸腺、脾脏指数,调节血清T-AOC、GSH-Px、LDH活性。结论滋补肾精方通过调节肾精不足证小鼠的体质量、胸腺和脾脏指数以及抗氧化能力发挥补肾填精作用。OBJECTIVE To observe the effect of Zibu Shenjing Fang on the mice with kidney essence deficiency,and explore the mechanism of nourishing kidney to enrich essence.METHODS 50 healthy male Kunming mice were divided into 5 groups： normal group,model group,Jingui Shenqi Pill group,Zibu Shenjing Fang high-dose,and low dose groups.The animal model of kidney essence deficiency was established by the method of terror impairing the kidney and over-fatigue for 21 days.From the start,mice in the model group were was given saline（0.02mL/g）,mice in Jingui Shenqi Pill group was given Jingui Shenqi pills [2.7g/（kg·d）],and mice in Zibu Shenjing Fang high-dose and low-dose groups were given different concentration of Zibu Shenjing Fang [20g/（kg·d）,10/（kg·d）] respectively for 21 days and then the general situation,the weight,the thymus and the spleen of mice,detected T-AOC,GSH-Px and LDH in serum were detected.RESULTS Zibu Shenjing Fang not only increases the indexes of the thymus and the spleen,but also improves the levels of T-AOC,GSH-Px and LDH in serum.CONCLUSION Zibu Shenjing Fang plays the role of nourishing kidney to enrich essence by increasing antioxidant value in mice with kidney essence deficiency.

关 键 词：滋补肾精方 肾精不足证 机理 

分 类 号：R285.5[医药卫生—中药学]