白豆杉细胞培养  

Cell culture of \%Pseudotaxus chinenii \%(Cheng) Cheng

作　　者：康强胜[1] 卢大炎[1] 李俊 李洪林[1] 吴玉兰[1] 

机构地区：[1]中国科学院武汉植物研究所,武汉430074

出　　处：《植物资源与环境》1999年第4期59-60,共2页Journal of Plant Resources and Environment

摘　　要：紫杉醇是从短叶红豆杉〔Ｔａｘｕｓｂｒｅｖｉｆｏｌｉａ(Ｃｈｅｎｇ)Ｃｈｅｎｇ〕树皮中分离出来的二萜类生物碱[1],现已成为知名抗癌药物。紫杉醇主要来源于红豆杉属植物。白豆杉〔Ｐｓｅｕｄｏｔａｘｕｓｃｈｉｅｎｉｉ(Ｃｈｅｎｇ)Ｃｈｅｎｇ〕属红豆杉科白豆杉属,为...Callus cultures of \%Pseudotaxus chienii \%(Cheng) Cheng were induced using different tissue explants including young stems and needles. The effecs of different concentrations and ratios of auxin and cytokinin on callus growth were analyzed. Cell suspensions of stem callus were grown for 30 days in shake flasks containing MS medium supplemented with 5 mg/L NAA, 1 mg/L BA and 3% sucrose. Through the growth cycle, fresh and dry weight accumulation, pH variation in the medium, and the uptake of sucrose was examined. The dry weight of the cell cultures approached its maximum value on the 25th day or so. A biomass yield of 16.67 g/L was obtained. Total carbohydrate uptake was closely associated with the increase in dry biomass. The average sucrose consumption rate was 0.83 g/L·d. The amounts of taxol in the cell were determined by reversephase high performance liquid chromatography. The average amount of taxol in the dry biomass was 9×10\+\{-8\}g/g.

关 键 词：白豆杉 细胞培养 紫杉醇 

分 类 号：S567.190.3[农业科学—中草药栽培]