Cloning and Analysis of WRKY Gene of Rice Induced by Rhizoctonia solani Kuhn  

纹枯病菌诱导水稻表达的WRKY基因克隆及分析(英文)

作　　者：姜述君[1,2] 马建[2] 范文艳[2] 戴凌燕[1] 张国庆[2] 于涵[2] 刘朝[2] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]黑龙江八一农垦大学农学院,黑龙江大庆163319

出　　处：《Agricultural Science & Technology》2011年第2期191-194,共4页农业科学与技术（英文版）

基　　金：Supported by Young Academic Backbone Support Program of Heilongjiang Province(1152G022)~~

摘　　要：[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by reverse transcription-polymerase chain reaction（RT-PCR）.Then RT-PCR products were cloned into the PMD18-T vector and sequenced.The functions of the sequence were predicted with bioinformatics method.[Result] A 1 079 bp gene was obtained.The gene encoded a protein with 236 amino acids.The protein contains many motif sites,two WRKY domains and a C2H2 zinc finger motif.The gene showed high identities with WRKY8,WRKY24 and WRKY30 gene of rice.[Conclusion] The up-regulated expression gene induced by R.solani was representative WRKY family gene.The gene could play an important role on rice sheath blight resistance.[目的]克隆纹枯病菌诱导的水稻上调表达基因。[方法]对应用抑制差减杂交技术(SSH)获得的纹枯病菌诱导水稻表达的EST序列K16进行电子克隆,根据电子克隆产物设计引物进行RT-PCR、克隆测序,利用生物信息学技术对克隆产物进行功能预测。[结果]克隆出一个长度为1079bp的序列,结构功能分析显示该基因编码236个氨基酸的蛋白,存在多个功能位点,含有2个典型的WRKY结构域和1个C2H2锌指型结构,该基因与水稻WRKY8、WRKY24和WRKY30基因有较高的同源性。[结论]经电子克隆获得的纹枯病菌诱导表达的基因为典型的WRKY家族基因,该基因可能在水稻抗纹枯病中起重要作用。

关 键 词：RICE Rhizoctonia solani Kuhn Silico cloning WRKY gene 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]