Cloning of H6H Gene from Atropa belladonna and Construction of the Efficient Plant Expression Vector  

颠茄H6H基因的克隆及高效植物表达载体的构建(英文)

作　　者：王贵君[1] 郑月[1] 陈敏[2] 廖志华[1] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]西南大学药学学院,重庆400715

出　　处：《Agricultural Science & Technology》2011年第2期208-210,共3页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Chongqing~~

摘　　要：[Objective] The aim was to clone H6H gene from Atropa belladonna and construct an efficient plant expression vector.[Method] The coding sequence of H6H（Hyoscyamine 6β-hydroxylase）was cloned from Atropa belladonna with RT-PCR.Then,the sequence was subcloned into the reconstructed plant binary expression vector p2301 to construct the recombinant vector p2301-H6H,which was then introduced into Agrobacterium tumefaciens strain LBA4404 and Agrobacterium rhizogenes strain C58C1,respectively.[Result] The engineering bacteria p2301-H6H-LBA4404 and p2301-H6H-C58C1 which could be directly used in genetic improvement were obtained.[Conclusion] The present research provided basis for the increasing of alkaloid content of Atropa belladonna by plant genetic engineering technology.[目的]克隆颠茄(Atropa belladonna)H6H基因并构建高效植物表达载体。[方法]采用RT-PCR方法从颠茄(Atropa belladonna)中克隆莨菪碱-6β-羟化酶和1,4-丁二胺-氮-甲基转移酶基因编码区,插入经改造后获得双元三价植物高效表达载体p2301-gus,构建植物表达载体p2301-H6H,并将该表达载体导入根癌农杆菌LBA4404和发根农杆菌C58C1。[结果]获得了可直接用于遗传改良的颠茄工程菌p2301-H6H-LBA4404和p2301-H6H-C58C1。

关 键 词：Atropa belladonna Hyoscyamine 6β-hydroxylase Plant expression vector 

分 类 号：S567.239[农业科学—中草药栽培]