Subcellular Localization of Aquaporins OsPIP2-6 in Rice  

水稻水通道蛋白OsPIP2-6的亚细胞定位研究(摘要)(英文)

作　　者：姚清国[1] 李晓芹[1] 周二鹏[1] 王娟[1] 张文娜[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《Agricultural Science & Technology》2011年第3期387-388,404,共3页农业科学与技术（英文版）

基　　金：Supported by Program for Science and Technology Development in Shijiazhuang City(10120803);Doctoral Research Fund of Shijiazhuang University(2007012)~~

摘　　要：[Objective] This study was to explore the subcellular localization of aquaporins OsPIP2-6 in rice. [Method] A key rice aquaporins gene OsPIP2-6 was cloned and used for construction of a transient expression vector,which was then transformed into onion epidermis via particle bombardment for confocal microscopy analysis using YFP gene as a reporter gene. [Result] The results showed that rice aquaporins OsPIP2-6 was mainly located in the plasma membrane. [Conclusion] Our results provided theoretical basis for further understanding plant aquaporins.[目的]探讨水稻水通道蛋白OsPIP2-6的亚细胞定位。[方法]克隆了水稻中的重要通道蛋白OsPIP2-6基因,构建了瞬时表达载体,通过基因枪法在洋葱表皮中进行瞬时表达,并通过激光共聚焦显微镜进行了观察。[结果]水稻水通道蛋白OsPIP2-6主要定位在细胞质膜上,证明了OsPIP2-6是个水通道蛋白。[结论]为植物水通道蛋白的深入研究提供了理论依据。

关 键 词：AQUAPORINS RICE Subcellular localization 

分 类 号：S511[农业科学—作物学]