RAPD Analysis for Genetic Diversity of Varanus salvator  

圆鼻巨蜥遗传多样性的RAPD分析(摘要)(英文)

作　　者：傅美兰[1,2] 于冬梅[2] 王莹[1] 彭建军[2] 

机构地区：[1]暨南大学生命科学技术学院,广东广州510632 [2]华南濒危动物研究所物种鉴定中心,广东广州510260

出　　处：《Agricultural Science & Technology》2011年第3期400-404,共5页农业科学与技术（英文版）

基　　金：Supported by Natural Science Foundation of Guangdong Province( 9151026001000003 );Outstanding Young Scientists Fund of Guangdong Province Academy ( 200704 );Wildlife Conservation and Utilization Public Laboratories Fund of Guangdong Province(200901)~~

摘　　要：[Objective] The aim was to carry out RAPD analysis on genetic diversity of Varanus salvator. [Method] 20 random primers were used for PCR amplification of genomic DNA of 36 individuals of V. salvator. [Result] 10 primers could produce highly reproducible RAPD bands. A total of 2 952 DNA fragments were successfully amplified. Each individual got 82 amplified bands on average,47 of which showed polymorphism. The polymorphic locus percent was 57.32%. The genetic distance among 36 individuals ranged from 0.035 9 to 0.335 9 with an average of 0.135 9. The Nei＇s gene diversity index （H） and Shannon＇s information index （I） were 0.181 9 and 0.263 0,respectively,indicating that V. salvator had greater genetic diversity. [Conclusion] The phylogenetic tree was inferred by using UPGMA analysis,it was found that the 36 individuals could be classified as one group,and there was no obvious population differentiation.[目的]采用RAPD技术对圆鼻巨蜥(Varanus salvator)进行遗传多样性分析。[方法]用20个随机引物对圆鼻巨蜥36个个体的基因组DNA进行PCR扩增。[结果]有10对引物能扩出清晰稳定的条带,共扩增出2952条DNA片段,平均每个个体扩增出82个条带,其中47条具多态性,多态性位点比为57.32%,36个个体间遗传距离为0.0359～0.3359,平均遗传距离为0.13592。Neis基因多样性指数H=0.1819,Shannons多样性指数I=0.2630,表明圆鼻巨蜥具有较高的遗传多样性。[结论]采用UPGMA法构建了36个个体相互关系的分子聚类图,发现没有形成明显的种群分化。

关 键 词：Varanus salvator Genetic diversity RAPD UPGMA analysis 

分 类 号：Q953[生物学—动物学]