Analysis on Polymorphisms of GHR Gene in Improved Hybrid Yellow Cattle Groups from Liupan Mountain Area in Ningxia  

宁夏六盘山区黄牛杂交改良群体GHR基因多态性分析(摘要)(英文)

作　　者：张春珍[1] 李聚才[2] 杨易[1] 于建勇 

机构地区：[1]宁夏大学,宁夏银川750021 [2]宁夏农林科学院,宁夏银川750002 [3]泾源县农牧与科技局,宁夏泾源756400

出　　处：《Agricultural Science & Technology》2011年第3期413-416,共4页农业科学与技术（英文版）

基　　金：Supported by Ningxia Science and Technology Program(5183003);Action Project for Scientific and Technological Personal to Service Enterprise (2009GJG30036)~~

摘　　要：[Objective] The paper aimed to analyze the polymorphism of the growth hormone receptor （GHR） in improved hybrid yellow cattle group from Liupan Mountain area in Ningxia Autonomous Region,so as to provide technological basis for hybrid improvement. [Method] Polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals. [Result] The target fragment of 338 bp was amplified. The PCR product digested by restriction enzyme Alu I showed polymorphisms. The frequencies of the two genotypes （AA,BB） were 75.71% （53 individuals） and 24.29% （17 individuals）,respectively. [Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle groups from Liupan Mountain area in Ningxia.[目的]对宁夏六盘山区黄牛杂交改良群体生长激素受体(GHR)基因的多态性进行分析,为宁夏黄牛杂交改良选育提供理论参考。[方法]采用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP),对该群体70个个体的GHR基因多态性进行检测。[结果]扩增出338bp的目的片段,PCR产物被限制性内切酶AluI消化后表现出多态性,其中AA基因型频率为75.71%;BB基因型频率为24.29%。[结论]共检测到AA和BB2种基因型,六盘山区黄牛杂交改良群体GHR基因具有多态性。

关 键 词：Hybrid yellow cattle group Growth hormone receptor gene POLYMORPHISM 

分 类 号：S823[农业科学—畜牧学]