Cloning of CBF3 Gene from Arabidopsis thaliana and Construction of Plant Expression Vector  

拟南芥CBF3基因克隆和植物表达载体的构建(英文)

作　　者：张春平[1,2] 束良佐[1] 张启军[3] 吕川根[3] 蔡小宁[2] 

机构地区：[1]安徽农业大学,安徽合肥230036 [2]南京晓庄学院,江苏南京211171 [3]江苏省农业科学院,江苏南京210014

出　　处：《Agricultural Science & Technology》2011年第5期670-673,共4页农业科学与技术（英文版）

基　　金：Supported by Cultivation for New Varieties of Genetically Modified Organisms Technology Projects(2008ZX08001-004);Key Projects of Nanjing Xiaozhuang University(2007NXY01);Natural ScienceFoundation for Jiangsu Province Universities(08KJD180011)~~

摘　　要：[Objective] The aim was to clone CBF3 gene from Arabidopsis thaliana and construct plant expression vector pCAMBIA1301-Rd29A-CBF3.[Method] CBF3 gene and stress-inducible promoter Rd29A were amplified from the genomic DNA of A.thaliana for the construction of plant expression vector.[Result] Sequencing results showed that the cloned CBF3 gene had 750 bp,and showed 100% identity with the sequence published on GenBank.The promoter Rd29A had 1 425 bp,and showed 100% identity with the sequence published on GenBank.[Conclusion] Based on the binary vector pCAMBIA1301,the plant expression vector pCAMBIA1301-Rd29A-CBF3 was constructed successfully,which could materially improve the salt resistance,drought-tolerance,cold resistance of plants.[目的]克隆拟南芥CBF3基因并构建植物表达载体pCAMBIA1301-Rd29A-CBF3。[方法]从模式植物拟南芥中克隆分离出转录因子CBF3与逆境诱导型启动子Rd29A的DNA序列,构建植物表达载体。[结果]克隆得到的CBF3与GenBank数据库所公布序列比对发现,同源性达到100%,全长为750bp;克隆克隆得到的Rd29A与GenBank数据库公布序列比对发现,同源性达到100%,全长为1425bp。[结论]以双元载体pCAMBIA1301为基础,植物表达载体pCAMBIA1301-Rd29A-CBF3被成功构建,对提高植物耐盐、耐旱和耐寒性有很大帮助。

关 键 词：CBF3 RD29A PROMOTER CLONE Plant expression vector 

分 类 号：Q943.2[生物学—植物学]