小鼠富亮氨酸重复结构蛋白Lrig2基因、蛋白结构及组织定位分析  

Structure and Tissue Localization Analysis of Mus Lrig2 Gene and Protein

作　　者：柴立民[1,2] 张园[2] 车永哲[2] 杨荣存[2] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室,北京100700 [2]南开大学医学院分子免疫学实验室,天津300071

出　　处：《现代生物医学进展》2011年第11期2014-2017,共4页Progress in Modern Biomedicine

基　　金：国家高科技术研究发展计划(863计划;No.2006AA020502)

摘　　要：目的:分析小鼠富亮氨酸重复结构蛋白家族成员Lrig2的基因与蛋白的结构,明确其组织分布和定位,并对其功能进行初步预测。方法:利用生物信息学分析技术对小鼠Lrig2基因的染色体定位、蛋白结构进行分析预测;通过RT-PCR、mRNA原位杂交技术检测Lrig2基因在小鼠不同组织中的表达定位;通过系统进化树分析Lrig2与其他Lrrs蛋白家族成员的同源性。结果:生物信息学分析显示,Lrig2是一种跨膜蛋白受体,是Lrrs蛋白超家族成员之一,胞外区含有15个Lrr模序、3个免疫球蛋白样结构域,存在单一的跨膜结构域;Lrig2在小鼠的多个组织中表达,其中在胸腺、脾脏等组织中表达较强;系统树分析显示,Lrigs蛋白是sLrPs超家族成员,是一种跨膜蛋白受体。结论:Lrig2在免疫组织中表达较强,推测其可能在肿瘤免疫应答进程中发挥重要的效能。Objective： To investigate the gene and protein structure ofMus Leucine-rich repeat immunoglobulin-like domain protein 2 （Lrig2）, and to definite its tissue distribution and localization, predict its biologic function. Methods： Bioinformatics analytical technique was used to analyze the chromosomal localization and protein structure of Mus Lrig2. The expression distribution of Lrig2 in mouse tissues was detected by RT-PCR and mRNA in situ hybridization. The phyletic evolution tree was used to analyze the homologous with Lrrs protein family. Results： Lrig2 was a transmembrane protein, which was one member of Lrr protein family. There were 15 Lrr motif and 3 immunoglobulin-like domains. This protein has one transmembrane domain. Lrig2 gene was expressed in several tissues, especially in thymus and spleen. Lrigs proteins belong to a new class of the sLrP family. It is a transmembrane protein. Conclutions： The expression of lrig2 in immunity tissues were notably, which indicated that Lrig2 played important biologic function in immunologic response of cancer.

关 键 词：Lrr-免疫球蛋白结构域样蛋白2 基因与蛋白结构 组织定位 

分 类 号：Q953[生物学—动物学] Q75