不同剂量甲泼尼龙对急性百草枯中毒大鼠早期肺损伤干预研究  

Effects of Different Doses of Methylprednisolone on Early Lung Injury in Paraquat Toxic Rats

作　　者：马玉英[1] 赵文鹏[2] 豆周林 王占青[2] 

机构地区：[1]石河子大学医学院第一附属医院急诊科,新疆石河子832008 [2]石河子大学医学院,新疆石河子832002

出　　处：《现代生物医学进展》2011年第11期2103-2106,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨不同剂量的甲泼尼龙(Methylprednisolone,MPS)对急性百草枯(paraquat,PQ)中毒大鼠早期肺损伤的疗效。方法:采用腹腔注射20%的PQ溶液制作大鼠急性PQ中毒的模型,随机均分为五组,正常对照组(A组)、染毒组(B组)、5mg/kg甲泼尼龙干预组(C组)、15mg/kg甲泼尼龙干预组(D组)、30mg/kg甲泼尼龙干预组(E组)。分三个不同时间点(24、72、168h)处死大鼠(每组每时间点6只)。观察各时间点大体标本,组织病理、肺系数和氧合指数。结果:光镜下肺组织病理学观察,与B组比较C、D、E组大鼠肺的病理学改变,肺泡腔内出血、渗出,炎性细胞浸润、肺泡隔炎性细胞浸润相对较轻,其中以C、E组减轻最为明显。在各组相同时间点肺系数:在三个时间点的值均比B组低(P<0.05),其中E组在24h、72h时间点上与C、D组有显著差异(P<0.05)。C、D、E组与B组的氧合指数的比较各个时间点上均与B组有差异(P<0.05),三组之间相互无明显差异。结论:本实验结果显示甲泼尼龙对急性百草枯中毒大鼠的肺损伤具有保护作用,且30mg/kg甲泼尼龙组要优于5mg/kg、15mg/kg甲泼尼龙组。Objective： To study the effect of different does of Methylprednisolone on early lung injury of rats with pamquat intoxication. Methods： 114 Wistar rats were randomly divided into 5 groups ： normal control group （A group）,intoxication group （B group）, 5mg/kg Methylprednisolone group （C group）, 15mg/kg Methylprednisolone group （D group） and 30mg/kg Methylprednisolone group（E group）. The 22mg/kg paraquat dissolvent was injected by peritoneal injection in B, C, D, E group for once. Then sacrificing the rats in 5 groups at 3 time points （24h ,72h ,168h）, describing lung tissue and observing oxygenation index and lung ratios and the lung pathological changes by light microscope. Results： C, D,E group compared with B group, the pathological changes of acute lung injury such as alveolar hemorrhage, exudation, inflammatory cell infiltration, alveolar septal infiltration of inflammatory cells are relatively mild.C and D Group are the lighter of pathological injury. In each group the same time in lung ratios： B group is the largest, group E in the24h and 72h points score was the lowest （P 〈0.05）. oxygenation index： C,D,E group are increase （P 〈0.05）. Conclusion： 30mg/kg Methylprednisolone has protective effect on lung injury of rats with paraquat intoxication and it is better than 5mg/kg and 15mg/kg Methylprednisolone.

关 键 词：甲泼尼龙 百草枯 急性中毒 肺损伤 

分 类 号：Q953[生物学—动物学]