小麦叶锈病生防菌株的筛选  

Screening of biocontrol agents for suppression of wheat leaf rust caused by Puccinia triticina

作　　者：王玉凤[1] 宋艳祥[2] 张汀[1] 刘大群[1] 冉隆贤[1,2] 

机构地区：[1]河北农业大学河北省农作物病虫害生物防治工程技术研究中心,河北保定071001 [2]河北农业大学林学院,河北保定071001

出　　处：《河北农业大学学报》2011年第3期12-17,23,共7页Journal of Hebei Agricultural University

基　　金：河北农业大学科研基金资助

摘　　要：为了获得小麦叶锈病的生物防治菌株,本试验利用从植物中分离的5株内生细菌(CN015r,CN017r,CN078,CN180,SH),JM218和WCS417r 2个根际细菌,WCS358的2种转基因菌株(WCS358::phl,WCS358::phz)以及龟裂链霉菌(CN124)和金色链霉菌(CN182)11个菌株作为供试菌,进行小麦叶锈菌夏孢子的萌发抑制试验,获得了7株对孢子萌发有较好抑制作用的菌株,对有效的菌株分别筛选标记其抗利福平的突变体菌株,配制成一定浓度的菌悬液,采用浸种和喷雾两种方法,对小麦叶锈病进行室内的防治试验。结果显示,经浸种处理后,CN180r菌株对小麦叶锈病具有明显的防效,严重度比对照下降了50.2%。在喷雾处理中,CN124对小麦叶锈病的防治效果显著,发病率下降了71.1%。经过分子鉴定,CN180测序后在NCBI中进行BLAST比对,与编号为EU761590.1菌株的相似性达97%,为桔黄假单胞菌(Pseudomonas aurantiaca),该菌能够在小麦体内定殖,也可以到达小麦的表面,获得了对小麦叶锈病有防治作用的内生细菌菌株。In order to obtain strains with potential biological control effects of wheat leaf rust,eleven bacterial strains,including five endophytic bacterial strains,CN015r,CN017r,CN078,CN180,and SH,two rhizosphere bacteria,JM218 and WCS417r,two genetically modified strains of WCS358,WCS358：：phl,WCS358：：phz,and two Streptomyces strains,S.rimosus CN124 and S.aureus CN182,were screened for their inhibitive ability to urediospore germination of Puccinia triticina,the pathogen of wheat leaf rust.And seven effective strains were obtained.The rifampcin resistant mutants of these effective strains were selected respectively by growing them on KB agar plates supplemented with increasing rifampcin concentration.These strains with antibiotics resistant marker were tested for their suppression effects against wheat leaf rust using seed soaking and leaf spraying of bacterial suspension.The results showed that seed soaking treatment in suspension of endophytic bacterial strain CN180r had obvious control effect against wheat leaf rust and the disease severity dropped 50.2 percent compared with the control.In spraying treatment,CN124 had more significant effect and the disease incidence dropped 71.1 percent.Endophytic strain CN180 was identified as Pseudomonas aurantiaca by molecular identification,with a sequence homology similarity of 97% to the strain EU761590.1 through BLAST in NCBI.It can be detected not only inside the tissue of root,stem and leaf,but also on the surface of the wheat seedlings.To our knowledge,this is the first time to obtain an endophytic bacterial strain with potential effects for control of wheat leaf rust.

关 键 词：小麦叶锈病 生物防治 内生细菌 浸种 喷雾 

分 类 号：S763.106[农业科学—森林保护学]