灰葡萄孢(Botrytis cinerea)对氟啶胺的敏感基线及对不同杀菌剂的交互抗性  

Baseline-sensitivity of Botrytis cinerea to fluazinam and cross-resistance against diverse fungicides

作　　者：陈治芳[1,2] 王文桥[2] 韩秀英[2] 张小风[2] 马志强[2] 刘颖超[1] 赵建江[2] 

机构地区：[1]河北农业大学植物保护学院农药系,河北保定071001 [2]河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000

出　　处：《河北农业大学学报》2011年第3期33-36,共4页Journal of Hebei Agricultural University

基　　金：农业公益性行业科研专项(200903033);“十一五”国家科技支撑计划项目(2006BAD08A03);河北省科技支撑计划项目(06220110D)

摘　　要：为了明确灰葡萄孢对新型杀菌剂氟啶胺的敏感性分布和该药剂是否与其它杀菌剂存在交互抗性,本试验采用菌丝生长速率法,测定了采自河北省和山东省不同地区的99株灰葡萄孢对氟啶胺的敏感性及其中48株灰葡萄孢对氟啶胺、嘧霉胺、多菌灵、异菌脲、乙霉威和啶酰菌胺的敏感性。结果表明,氟啶胺对从未使用过氟啶胺的地区采集的灰葡萄孢99个野生菌株对氟啶胺的敏感性（EC50值）分布在0.001 4~0.030 3μg/mL之间,平均为（0.0151±0.007）μg/mL,其敏感性频率分布呈连续型单峰曲线,符合正态分布,可作为敏感基线,用于灰葡萄孢对氟啶胺田间抗性监测。在6种供试杀菌剂中氟啶胺对灰葡萄孢的毒力最高,其与嘧霉胺、异菌脲、多菌灵、乙霉威和啶酰菌胺之间均不存在交互抗性关系。Sensitivity of 99 strains of Botrytis cinerea collected from different regions of Hebei and Shandong provinces to fluazinam and sensitivity of 48 isolates to fluazinam,pyrimethanil,carbendazim,iprodione,diethofencarb and boscalid was determined by mycelial growth rate test to explicit sensitivity of B.cinerea to novel fungicide fluazinam and to make sure if there existed cross-resistance between fluazinam and other 5 botryticides.Mean EC50 values for 99 isolates from the areas where fluazinam has never been used before were（0.015 1±0.007） μg/mL,ranging from 0.001 4 to 0.030 3 μg/mL.The sensitivity frequency of B.cinerea strains to fluazinam distributed as a unimodel curve.Thus,it could be used as baseline-sensitivity of B.cinerea to fluazinam which could be used for field resistance monitoring.Fluazinam possesses higher inhibitory activity to mycelial growth of 6 tested isolates than the other 5 botryticides,and no positively-correlated cross resistance existed between fluazinam and the other 5 botryticides with different mechanism of action.

关 键 词：灰葡萄孢 杀菌剂 氟啶胺 敏感基线 交互抗性 

分 类 号：S482.2[农业科学—农药学]