巢式RT-PCR法检测HIV-1阳性者中庚型肝炎病毒感染的研究  

The study of HGV infection in HIV-positive population by nested RT-PCR

作　　者：徐萌[1] 绳波[1] 刘志英[1] 陈德喜[1] 吴昊[1] 

机构地区：[1]首都医科大学附属北京佑安医院性病艾滋病实验室,100069

出　　处：《北京医学》2011年第6期467-469,共3页Beijing Medical Journal

基　　金：男男性接触队列筛查工作模式及HIV急性期感染者管理体系研究(D09050703590901);国家自然科学基金(30872226);国家自然科学基金(30770742)

摘　　要：目的调查HIV流行地区庚型肝炎病毒(HGV)在HIV阳性人群中的感染率,并探讨其对HIV病程的影响。方法根据已知并公认的HGV序列,设计特异巢式引物,建立HGVRNA的巢式RT-PCR方法,对100例HIV-1阳性感染者进行检测,同时进行CD4+T细胞计数和HIV病毒载量的测定。结果 100例HIV-1阳性者中检测出HGVRNA阳性感染者22例(22%);合并感染HGV的HIV感染人群体内可见有较高CD4细胞数。结论 HIV阳性感染者有较高的HGV重叠感染率,可能与其具有共同的传播途径有关,合并感染HGV似乎能延缓HIV感染者的病程。Objective To Investigate the rate of hepatitis G（HGV） infection in HIV-positive population,HIV-endemic areas,and to explore its impact on HIV disease.Methods According to the known and recognized HGV sequence,we designed nested primers specifically at the 5＇ UTR region of HGV and established an effective methods to detect HGVRNA with nested RT-PCR,100 cases of HIV-1 positive people were detected,while the CD4＋ T cell count and HIV viral load were determined at the same time.Results Twenty-two cases（22%） were HGVRNA positive in 100 cases of HIV positive population detected;HGVRNA positive subjects among HIV-infected individuals have a higher number of CD4 cells.Conclusions The prevalence of HGV among HIV positive subjects was high,as a consequence to the a common transmission pathways,HGV infection seems to delay the progress of HIV disease.

关 键 词：庚型肝炎病毒 人类免疫缺陷病毒 聚合酶链式反应 供血 

分 类 号：R512.91[医药卫生—内科学] R512.6[医药卫生—临床医学]