CTLA4Ig基因在糖尿病大鼠胰岛移植中的作用  

THE EFFECT OF CTLA4IG GENE ON PANCREATIC ISLET ALLOGRAFT FOR DIABETIC RAT

作　　者：金世龙[1] 顾红光[1] 王亚旭[1] 刘宝华[1] 王代科[1] 文亚渊[1] 王仁云[1] 王东[1] 周月庆[1] 刘萍[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所普外科,重庆400042

出　　处：《中国普外基础与临床杂志》1999年第6期328-330,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：全军95 医药卫生科研基金

摘　　要：为探讨融合蛋白（ＣＴＬＡ４Ｉｇ） 基因在糖尿病大鼠体内表达及其产物延长胰岛移植物存活的作用，利用脂质体（Ｌｉｐｏｆｅｃｔｉｎ） 载体包裹ＣＴＬＡ４ＩｇｃＤＮＡ 质粒后转染鼠胰岛和肌肉细胞，检测移植后ＣＴＬＡ４Ｉｇ 基因表达水平和Ｔ 淋巴细胞转化率，观察ＣＴＬＡ４Ｉｇ 基因表达在延长糖尿病鼠胰岛移植物和大鼠存活时间的作用。结果： 胰岛移植术后实验（ Ａ） 组、对照（Ｂ） 组Ｔ 淋巴细胞转化率有明显差异（ Ｐ＜ ０ ．０５） 。Ａ 组胰岛移植后第７ 天２ 只大鼠血清ＣＴＬＡ４Ｉｇ 呈阳性（ 阳性率为２０ ％ ） ，分别为１４ ｎｇ／ ｍｌ 和３１ ｎｇ／ ｍｌ。Ｂ 组胰岛移植后维持血糖正常时间平均３ ．６ ±５ ．１ 天，Ａ 组胰岛移植后维持血糖正常时间平均１４ ．８ ±１２ ．３ 天，两组比较差异有显著性意义（ Ｐ＜ ０ ．０５ ） 。Ａ 组大鼠平均存活２４ ．０ ±１０ ．８ 天，最长４５ 天，Ｂ 组大鼠平均存活１０ ．８ ±４ ．８ 天，最长２１ 天，两组大鼠生存时间差异有统计学意义（ Ｐ＜ ０ ．０１） 。本实验结果提示：ＣＴＬＡ４Ｉｇ 基因可以在受体大鼠胰岛细胞或肌肉组织表达，其表达产物可使胰岛移植物和受体鼠存活时间明显延长。To study the expression of CTLA4Ig gene in diabetic rat and the effect of CTLA4Ig on long term survival of the pancreatic islet. The rat pancreatic islet cell and muscle cell transfected with the cDNA for CTLA4Ig packaged with lipofectin vector. We examined the expression level of CTLA4Ig gene, T lymphocyte reaction and observed the expression of CTLA4Ig cDNA in diabetic rat and the action of CTLA4Ig in long term survival of pancreatic islet transplanted and the transplanted rats. Results: The T lymphocyte reaction from peripheral intravenous blood at seventh day after surgery, the difference between two group was significant ( P <0.05). Only 2 out of 10 recipients of the experiment group (A) at the seventh day after pancreatic islet allograft had any detectable levels of CTLA4Ig, their concentration was 14 ng/ml, and 31 ng/ml. The average time of maintaining blood glycose in normal levels of the group A after pancreatic islets graft, 14.8±12.3 days, was significantly longer than 3.6±5.1 days of the control group (B) ( P <0.05). The average survival time of the group A, 24.0±10.8 days (the longest time was 45 days), was significantly longer than 11.8±4.8 days (the longest time was 21 days) of the group B ( P <0.01). Conclusions: The muclse cells and pancreatic islets of the recipient rat was transfected with CTLA4IgcDNA packaged with lipofectin, and CTLA4IgcDNA was expressed in recipient tissue, its expressed product CTLA4Ig make pancreatic islets transplanted and recipient rat survive longer significantly.

关 键 词：胰岛移植 基因治疗 糖尿病 CTLA4IG 

分 类 号：R657.505[医药卫生—外科学] R587.105[医药卫生—临床医学]