番茄Sly-miR167a启动子的分离及功能分析  

Isolation and Function Analysis of Sly-miR167a Promotor in Tomato

作　　者：苗青[1] 李正国[1] 杨迎伍[1] 李季[1] 胡国建[1] 

机构地区：[1]重庆大学生物工程学院基因工程研究中心重庆市高校功能基因与调控新技术重点实验室,重庆400030

出　　处：《热带作物学报》2011年第4期653-657,共5页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.30972002;No.31071798);重庆市自然科学基金重点项目(No.CSTC;2011BA1024);中央高校基本科研业务费(No.CDJXS)资助

摘　　要：利用PCR技术从番茄基因组DNA中扩增了Sly-miR167a转录起始点上游2 134 bp的片段,并将其与GUS基因融合构建植物表达载体,转化番茄,研究番茄Sly-miR167a启动子的时空表达特异性;并利用外源激素处理,研究其对激素的响应特性。启动子序列分析结果表明,Sly-miR167a启动子片段含有启动子调控的保守序列以及生长素、乙烯和赤霉素等响应元件。根据GUS染色结果推测,Sly-miR167a可能在番茄的各个组织中均有表达,并且在叶片、茎、开花前2d、开花当天、果实幼果期、绿熟期和破色期表达强烈。通过外源激素处理后,GUS基因的表达均有显著的上调。说明Sly-miR167a启动子的表达具有时间特异性,并且受外源激素的调控。In order to study the regulation of Sly-miR167a promotor, a 2 134 bp fragment upstream the transcription start was amplified from tomato genomic DNA by PCR, which was fused to GUS and transformed into tomato （cv Microtom）. The temporal and spatial specificity during plant development were analyzed. To investigate the response to exogenous hormone, treatments were performed on the transgenic tomato. Promotor analysis revealed that some conserved regulatory sequences and several regulatory elements appeared in the promotor fragment. The results indicated that Sly-miR167a might express in all tissues, and express intensely in leaves, stems, flowers at anthesis and 2 days before anthesis, and fruits at immature green, mature green and breaker stages, and it was up-regulated by ethylene, NAA and GA. This result revealed that the expression of Sly-miR167a promotor showed temporal and spatial specificity, and it was regulated by exogenous hormone.

关 键 词：Sly—miR167a 启动子 GUS 番茄 

分 类 号：S641.2[农业科学—蔬菜学] Q78[农业科学—园艺学]