海马齿SRTG152-I基因表达产物的亚细胞定位  

Subcellular Localization of Expression Products of SRTG152-I Gene From Sesuvium portulacastrum

作　　者：段瑞军[1] 符少萍[1] 郭建春[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《热带作物学报》2011年第4期668-672,共5页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(310081);中央级公益性科研院所基本科研项目(ITBB110217)

摘　　要：SRTG152-I基因是从盐生植物海马齿中分离的与K+离子抗性相关的新基因。本研究将SRTG152-I基因的编码区与mgfp荧光蛋白基因融合,定向克隆至植物表达载体pCAMBIA1304上,构建SRTG152-I基因植物瞬时表达载体。用基因枪法将该表达载体导入洋葱表皮细胞,然后在荧光显微镜下观察其在洋葱表皮细胞中的亚细胞定位,结果表明,SRTG152-I基因表达蛋白主要分布在洋葱表皮细胞膜或细胞壁上,说明该蛋白是一个与K+离子抗性相关的膜蛋白。SRTG152-1 is a new gene, which relates to K^＋ tolerance and is isolated from the halophyte S. portulaccastrum. In this research, the open reading frame of SRTG152-1 was translationally fused with GFP gene, then inserted to plant expression vector pCAMBIA1304. The expression vector pCAMBIA1304 was transformed into onion epidermal cells by particle bombardment method. The suh-cellular localization of the gene-product was observed in onion epidermal cells under fluorescent microscope. The result showed that the expression product of SRTG152-1 was located at the onion membrane or cell wall. Thus, it is deduced that SRTG152-1 is a K^＋- resistant membrane protein.

关 键 词：SRTG152-I基因 亚细胞定位 植物表达载体 

分 类 号：Q78[生物学—分子生物学]