双孢蘑菇营养菌丝老化现象的形态学研究  

Morphological studies on senescence of vegetative mycelium of Agari cus bisporus

作　　者：王博[1] 许修宏[1] 袁立[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《东北农业大学学报》2011年第5期99-103,共5页Journal of Northeast Agricultural University

基　　金：国家十一五科技支撑项目(2008BADA1B01);中国科学院知识创新工程重大项目(KSCX1-YW-09-09-03);哈尔滨市科技攻关计划项目(2007AA6CN105);东北农业大学创新团队发展计划项目(CXT003-2-1)

摘　　要：通过观察双孢蘑菇菌丝体的显微结构和检测菌丝体活力,找到双孢蘑菇营养菌丝老化的基本特征,描述了双孢蘑菇(Agaricus bisporus)的营养菌丝在不同时期的形态学变化。结果发现,在接种50～60d后菌丝开始老化,找到老化的标志性特征为细胞发生肿胀,产生液泡状物质;细胞壁变得粗糙不规则。结果表明,菌丝的萌发时间随着菌龄增大而增大,菌丝长速会随着菌龄增大而减缓;随着菌龄的增加菌丝的RNA含量降低。为今后鉴别生产中菌种的老化问题提供科学依据。By observing the microstructure of the vegetative mycelium of Agaricus bisporus and detecting the activity of mycelium,it would find that essential characteristics of the aged vegetative mycelium of Agaricus bisporus.The investigation showed that the morphological changes occurred in ageing vegetative mycelium of Agaricus bisporus in different periods.The results showed that the period of senescence occured from 50 to 60 days after inoculation.The sign of senescence was swelling and vacuole-like in the mycelium and the cell walls became coarse and irregular.The results showed that the mycelial growth rate decreased as the time of mycelial germination increased,and the content of RNA decreased while spawn age grew more older.For the identifying senescence problems of the production of mushroom spawn in the furture.

关 键 词：菌丝老化 形态学观察 菌丝活力 

分 类 号：S646[农业科学—蔬菜学]