兔抗鹅IgG酶标抗体的制备及初步应用  

Preparation of Rabbit Anti-goose IgG-HRP and Preliminary Application

作　　者：刘菲[1,2] 唐卫杰 韩清林[1,3] 赵婷婷[1] 高丽芹[1] 严晓玲[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]动物疫病与人类健康四川省重点实验室,四川雅安625014 [3]四川省大英县畜牧局,四川大英629300

出　　处：《中国畜牧兽医》2011年第6期91-94,共4页China Animal Husbandry & Veterinary Medicine

基　　金：四川省教育厅自然科学基金重点项目(2006A012);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848);现代农业产业技术体系建设专项资金资助(nycytx-45-12)

摘　　要：为了研究兔抗鹅IgG酶标抗体的制备,试验采用水稀释-辛酸-硫酸铵法粗提,透析除盐,DEAEA-50纤维素层析相结合的方法提取鹅卵黄IgG,经核酸蛋白检测仪和SDS-PAGE测定IgG浓度;制备兔抗鹅IgG酶标抗体;利用酶标抗体检测小鹅瘟病毒(GPV)VP3基因疫苗的免疫效果。结果表明,粗提的IgG浓度为10 mg/mL,抗体纯度可达到94.5%;免疫扩散得出兔抗鹅IgG抗血清效价约1∶32,抗体酶标后效价为1∶3000;酶标抗体可以作为检测疫苗效果一种很好的工具。To study on preparation of rabbit anti-goose IgG-HRP,the experimentation isolated IgG from the goose egg yolk,and the method was employed to dilute with water-n-octylic acid-ammonia sulfate initial extraction,out salt dialysis and DEAEA-50 chromatography.Protein nucleic acid detector and SDS-PAGE indicated the IgG concentration;the purified IgG was labeled with HRP;effect of GPV VP3 gene vaccine was detected with the labeled antibody.The results indicated that the initial extraction was 10 mg/mL and the purity was 94.5%;the antiserum characterized by immunodiffusion showed that the titer of the antiserum was 1∶32 and specificity of the antiserum was high.The titer of labeled antibody was 1∶3000;the labeled antibody was a good tool to detect the effect of GPV VP3 gene vaccine.

关 键 词：鹅卵黄IgG 提取 酶标 小鹅瘟VP3基因疫苗 

分 类 号：S852.4[农业科学—基础兽医学]