百宜黑鸡与兴义矮脚鸡线粒体DNA控制区遗传多样性分析  

Genetic Diversity in Baiyi Black Chicken and Xingyi Bantam by Analyzing the mtDNA Control Region

作　　者：王文涛[1] 陈彬[1] 阳光远 傅筑荫[1] 

机构地区：[1]贵州大学,高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳550025 [2]贵州省现代农业发展研究中心,贵州贵阳550006

出　　处：《中国畜牧兽医》2011年第6期126-128,共3页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省科技厅攻关项目“贵州特色优质鸡新品种(系)的培育及综合配套技术的研究”[黔科合NY字(2007)3019]

摘　　要：采用PCR和直接测序的方法测定百宜黑鸡和兴义矮脚鸡线粒体DNA控制区全序列,比较分析两种贵州地方鸡种的遗传变异。结果表明,百宜黑鸡、兴义矮脚鸡mtDNA控制区全序列长均为1231 bp;共发现26个变异位点(不包含种内变异位点),占分析位点总数的2.11%,其中12个转换,14个颠换;百宜黑鸡mtDNA控制区的A、T、G、C碱基含量分别为27.096%、33.628%、13.117%、26.159%,矮脚鸡的A、T、G、C碱基含量分别是A26.916%、T33.409%、G13.387%、C26.288%,t检验结果显示,两种鸡mtDNA控制区的A和T含量差异显著。百宜黑鸡和兴义矮脚鸡的遗传距离是0.0379,根据分子钟计算,二者约在190万年前分歧进化。PCR and direct sequencing methods were used to analyze the entire mtDNA D-loop in Baiyi Black chicken and Xingyi Bantam.The D-loop of Baiyi Black chicken and Xingyi Bantam was 1231 bp,there were 26 variational sites,including 12 transitions and 14 transversions.The nucleotide composition of Baiyi Black chicken was A 27.096%,T 33.628%,G 13.117%,C 26.159%,and A 26.916%,T 33.409%,G 13.387%,C 26.288% of Xingyi Bantam.The genetic distance between two partridges was 0.0379.According to the calibrated rates of molecular evolution,they speciated about 1.9 million years ago.

关 键 词：百宜黑鸡 兴义矮脚鸡 线粒体DNA控制区 遗传多样性 

分 类 号：S831[农业科学—畜牧学]