几种检测猪弓形虫病ELISA诊断试剂盒的对比  

Comparison of Five ELISA Kits for Detection of Anti-Toxoplasma gondii Antibodies in Experimentally Infected Swine

作　　者：何勇[1] 朱艳平[2] 袁子国[1] 尹创成[1] 周东辉[1] 林瑞庆[1] 朱兴全[3] 翁亚彪[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]新乡学院,河南新乡453003 [3]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046

出　　处：《中国畜牧兽医》2011年第6期218-221,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30901067);高等学校博士学科点专项科研基金(20094404120016);广东省科技计划项目(2009B020307011);广东省自然科学基金(10451064201005443);广东省高等学校自然科学基金(9451064201003715);畜疫病病原生物学国家重点实验室(SKLVEB2009KFKT014;SKLVEB2010KFKT010);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)资助

摘　　要：以人工感染弓形虫的猪阳性血清及感染前的阴性血清为样品,比较3个国产(A、B、C)和2个国外(D、E)ELISA试剂盒检测猪弓形虫病的敏感性。用这5种ELISA试剂盒,检测人工感染弓形虫的猪阳性血清42份及感染前的阴性血清45份,并参考PCR扩增结果,比较这5种ELISA试剂盒检测猪弓形虫病的敏感性。5种试剂盒中,1种国产试剂盒的检测结果与PCR结果完全一致,其余4种试剂盒的检测结果都与PCR检测结果差别较大。结果表明,国产试剂盒A的检测效果最佳,国外试剂盒与部分国内试剂盒的检测结果相当,临床选用试剂盒时,可选择检测效果最佳的试剂盒A,不必盲目选择国外试剂盒。The aim of the present study was to evaluate and compare the sensitivities of five ELISA kits for detection of Toxoplasma gondii infection in experimentally infected pigs.The PCR-positive serum samples from experimentally infected pigs were examined by the five ELISA kits.The results of different ELISA kits were compared among them and with that of PCR amplification to evaluate and compare their sensitivities.One ELISA kit（ELISA-A） made in China showed 100% detection of T.gondii antibody in experimentally infected pigs,showing 100% sensitivity.Whereas,the other ELISA kits displayed different low sensitivities.These results indicated that the ELISA-A kit is the most sensitive for detection of T.gondii infection in pigs.

关 键 词：弓形虫 猪弓形虫病 人工感染 ELISA 对比 

分 类 号：S858.28[农业科学—临床兽医学]