常山提取物急性毒性试验研究  

Study on the Acute Toxicity of Dichroa Febrifuga Extractive

作　　者：雷宏东[1] 梁剑平[2] 郭志廷[2] 王学红[2] 华兰英[2] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050

出　　处：《中国畜牧兽医》2011年第6期236-238,共3页China Animal Husbandry & Veterinary Medicine

基　　金："十一五"国家科技支撑计划项目(2006BAD31B05)

摘　　要：为了评价常山提取物的安全性,对其进行了小白鼠的急性毒性试验。预试验初步确定用药剂量,正式试验取60只昆明种小白鼠,灌胃给药后,连续观察7 d,记录小白鼠的急性毒性反应,并通过改良寇氏法计算常山提取物对小白鼠的半数致死量（LD50）及LD50的95%可信限。结果表明,给药组部分小白鼠出现中毒反应并死亡,剖检主要脏器未见病理变化,测得LD50为18.16 g/kg体重,LD50的95%可信限为15.35~21.49 g/kg体重。试验结果初步提示,常山提取物的毒性很低,临床用药安全可靠。In order to evaluate the security of dichroa febrifuga extractive,the acute toxicity of dichroa febrifuga extractive was tested in mice.After preliminary experiment to determine the dosage,60 mice were selected and divided into 6 groups.After giving medicine lavage and observing for 7 days,the acute toxic reaction was recorded and the LD50 value and 95% confidence limit of LD50 were determined by the modified Karber＇s method.The results showed that some mice appeared toxic reaction and death.No pathological change was found in the main organs by necropsy of dead mice.The LD50 was 18.16 g/kg body weight and its 95% confidence limit was 15.35 to 21.49 g/kg body weight.The results demonstrated that the dichroa febrifuga extractive has lower toxicity and safety for clinical application.

关 键 词：常山提取物 急性毒性 小白鼠 

分 类 号：S859.8[农业科学—临床兽医学]