气相色谱法测定香附、苍术挥发油中α-香附酮的含量  

Determination of α-Cyperone in Volatile Oil Extracted from Rhizoma cyperi and Rhizoma atractylodis by Gas Chromatography

作　　者：吴凤英[1] 游本刚[1] 唐丽华[1] 缪刚[2] 许奇[2] 王业东[1] 

机构地区：[1]苏州大学医学部药学院药剂学系,江苏苏州215123 [2]苏州药品检验所,江苏苏州215002

出　　处：《苏州大学学报（医学版）》2011年第2期250-252,275,共4页Suzhou University Journal of Medical Science

基　　金：2010年苏州大学大学生创新性实验计划项目基金(5731512910)

摘　　要：目的建立测定香附、苍术挥发油中α-香附酮含量的气相色谱方法。方法运用超临界CO2萃取法同时提取香附、苍术中的挥发油,并通过气相色谱法测定油中α-香附酮含量。结果α-香附酮在319.20～1 596.00μg/ml范围内线性关系良好(r=0.9991,P<0.05)。结论气相色谱法准确、简单,可用于香附、苍术混合挥发油中α-香附酮含量测定。Objective To establish a gas chromatography method for the determination of α-cyperone in the volatile oil extracted from Rhizoma cyperi and Rhizoma atractylodis.Methods The volatile oil was extracted from Rhizoma cyperi and Rhizoma atractylodis by supercritical CO2 fluid extraction.Results A good linearity of α-cyperone was obtained in the concentration range from 319.20 to 1596.00 μg/ml（r=0.9991,P0.05）.Conclusion The method is rapid and stable,and can be used for determining the content of α-cyperone in the mixed volatile oil.

关 键 词：气相色谱 香附 苍术 挥发油 Α-香附酮 

分 类 号：R927.2[医药卫生—药学]