耐亚胺培南鲍氏不动杆菌碳青霉烯酶表型与基因型研究  

Study on carbapenemase phenotype and genotype in imipenem-resistant acinetobacter baumannii

作　　者：陈柳勤[1] 侯铁英[2] 孙诚[3] 张莉滟[1] 陈晓丽[1] 黄爱伟[1] 

机构地区：[1]广东省人民医院广东省医学科学院临床微生物室,广东广州510080 [2]广东省人民医院广东省医学科学院医院感染管理科,广东广州510080 [3]广东省人民医院广东省医学科学院急危重症医学部,广东广州510080

出　　处：《中华医院感染学杂志》2011年第12期2389-2391,共3页Chinese Journal of Nosocomiology

摘　　要：目的研究某综合医院临床分离耐亚胺培南鲍氏不动杆菌碳青霉烯酶基因型携带状况,为临床合理用药及医院感染控制提供依据。方法对2008年1月-2009年10月临床分离的100株耐亚胺培南鲍氏不动杆菌,应用聚合酶链反应检测其金属β-内酰胺酶的耐药基因:blaIMP、blaVIM、blaSIM-1和OXA型碳青霉烯酶的耐药基因:blaOXA-23、blaOXA-24。结果所有受试验菌株均未检测到金属β-内酰胺酶耐药基因blaIMP、blaVIM、blaSIM-1和OXA-24型碳青霉烯酶的耐药基因,PCR检测到79株菌携带blaOXA-23,在阳性菌株中随机选取2株测序,在网上与NCBI在线数据库的已知产OXA-23碳青霉烯酶鲍氏不动杆菌进行比对,与登录号EF534259.1的同源性为100.0%。结论某综合医院临床分离耐亚胺培南鲍氏不动杆菌未产金属β-内酰胺酶,主要耐药机制是产OXA-23型碳青霉烯酶。OBJECTIVE To study the genotype of the imipenem-resistant Acinetobacter baumanniis（IRAB） isolated from clinic in one general hospital for rational use of antibiotics and control of nosocomial infections.METHODS PCR was used to detect gene of metallo-β-lactamases,such as blaIMP,blaVIM and blaSIM-1;gene of OXA-like carbapenemase,such as blaOXA-23、blaOXA-24.RESULTS Gene of blaIMP,blaVIM,blaSIM-1,blaOXA-24 were not found in all the isolates.79 strains possed gene blaOXA-23.2 strains were randomly selected from the 79 clinical isolates to sequence.The comparison of the sequence with all the other known members of blaOXA-23 showed that they shared the highest identity（100%） with GenBank,EF534259.1 from NCBI Online database.CONCLUSION IRAB isolated from clinic in one general hospital do not produce metallo-β-lactamases,OXA-23 carbapenemase is the main mechanism of resistance.

关 键 词：鲍氏不动杆菌 金属Β-内酰胺酶 OXA型碳青霉烯酶 亚胺培南 聚合酶链反应 

分 类 号：R378[医药卫生—病原生物学]