补肾益气活血方对宫内生长迟缓胎儿一氧化氮及脂质过氧化水平的影响  

Effects of the Prescription of Reinforcing Kidney, Replenishing Qi and Promoting Blood Circulation on the Umbillical Serum Level of Nitric Oxide and Lipid Peroxidation in Intrauterine Growth Retardation

作　　者：舒益民[1] 黄光英[1] 原本旭[2] 乔福元[2] 胡道松[3] 

机构地区：[1]同济医科大学附属同济医院.同济医科大学中西医结合研究所,邮政编码430030 [2]同济医科大学附属同济医院妇产科 [3]同济医科大学神经生物学教研室

出　　处：《微循环学杂志》1999年第3期14-16,共3页Chinese Journal of Microcirculation

基　　金：国家自然科学基金!39870923;国家中医药管理局科研基金!922204

摘　　要：探讨补肾益气活血方对胎儿宫内生长迟缓（ＩＵＧＲ）胎盘微循环障碍的影响。方法：应用ＮＡＤＰＨ一黄递酶组织化学方法检测胎盘组织一氧化氮合酶（ＮＯＳ）活性，采用镇铜镉还原法及硫代巴比妥酸比色法（ＴＢＡ法）测定了新生儿脐血血浆亚硝酸盐／硝酸盐（ＮＯ２－／ＮＯ３－）和丙二醛（ＭＤＡ）浓度。结果：ＩＵＧＲ孕妇胎盘组织ＮＯＳ活性较正常足月妊娠明显下降，新生儿脐血ＮＯ２－／ＮＯ３－低于正常新生儿（<０．０１），而ＭＤＡ水平高于正常（Ｐ<０．０１），中药治疗后与正常对照组差异无显著性（Ｐ均＞０．０５），相关性分析表明ＮＯ２－／ＮＯ３－与ＭＤＡ呈明显负相关（ｒ＝－０．６１２４，Ｐ<０．０５）。结论：脂质过氧化可能是引起ＩＵＧＲ胎盘微循环障碍的中介因子之一，补肾益气活血方促进胎儿宫内生长发育与其抗自由基损伤，增强胎盘组织一氧化氮（ＮＯ）合成和分泌有关。The effects of the prescription of reinforcins kidney, replenishing qi andpromoting blood circulation (RKRQPBC) on placenta microcirculatory disturbance in intrauterine grouth retardation (IUGR ) were observed. NADPH--diaphorase histochemical method was used to study nitric oxide synthase(NDe) activity. A copper coated cerium reduction method and thiobarbituric acid(TBA)reaction were respectively adopted to measure the umbillical plasma concentration of NO2- /NO3- and malondialdehyde (MDA ). The results showed that theNOs activity of placental tissues in IUGR group was significantly weaker thanthat in normal control growth. The umbillical plasma level of NO2/NO3 in IUGR group was much lower(P<0. 05) and the level of MDA was much higher(P<0. 01 ) than that in normal control group. There was negative correlationbetween the level of NO2-/NO3- and MDA(r=-0. 612 4, P< 0. 05). Thedifference between Chinese medicine group and normal control group was not sig-nificant(p>0. 05) after treatment with the prescription of RKRQPBC. Thesesuggested that lipid peroxidation may be a crucial mediator of placenta mictocirculatory disturbance. The results indicated that the prescription of RKRQPBCcan enhance the growth of fetuses by way of preventing the injury of lipid peroxidation and raising the synthesis and release of nitric oxide in placental tissues inIUGR.

关 键 词：胎儿 宫内生长迟缓 补肾益气活血 一氧化氮 

分 类 号：R271.944.3[医药卫生—中西医结合]