不同产地枸杞药材中多糖的含量测定  

Content determination of polysaccharides in Lycium barbarum L.from different areas

作　　者：刘万仓[1] 孙磊[2] 乔善义[2] 王英华 王金辉[1] 

机构地区：[1]沈阳药科大学中药学院基于靶点的药物设计与研究教育部重点实验室,沈阳110016 [2]军事医学科学院毒物药物研究所九室,北京100850 [3]宁夏药品检验所,银川750004

出　　处：《国际药学研究杂志》2011年第3期229-231,共3页Journal of International Pharmaceutical Research

基　　金：国家科技支撑计划课题(2006BAI08B03-08);国家科技重大专项课题(2009ZX09301-002;2009ZX09103-361)

摘　　要：目的建立枸杞多糖的含量测定方法,并测定不同产地枸杞药材中多糖的含量。方法采用水提醇沉法制备枸杞多糖,将枸杞多糖脱蛋白后制得精制枸杞多糖,以精制枸杞多糖测得枸杞多糖对葡萄糖的换算因子后,用苯酚-硫酸法测定不同产地枸杞药材中多糖的含量。结果平均回收率为98.8%,测得不同产地枸杞药材中多糖含量分别为4.03%、7.55%、2.92%、4.99%、3.54%和4.67%。结论该法操作简单,结果较接近真实值;不同产地枸杞中多糖含量的差异较大,该结果为枸杞多糖质量控制和药效评价的进一步研究奠定了基础。Objective To establish a method for polysaccharides content determination and determine the content of Lycium barbarum L.polysaccharides（LBP） form different areas.Methods LBP were prepared by water extraction-alcohol precipitation method,and then deproteinized to obtain refined LBP.After the conversion coefficient of refined LBP to glucose was obtained,the content of LBP was determined by phenol-sulfuric acid method.Results The polysaccharide content of six batches LBP was 4.03%,7.55%,2.92%,4.99%,3.54% and 4.67%,respectively,and the average recovery rate reached 98.8%.Conclusion The method is simple,and the results are precisely close to the true value.The polysaccharide content of LBP from different areas is comparatively different,which lays a foundation for the further study of quality control and efficacy evaluation of Lycium barbarum L..

关 键 词：枸杞 多糖 含量测定 换算因子 

分 类 号：R284.1[医药卫生—中药学]