安多霖对微波辐射致大鼠海马细胞色素C氧化酶变化的影响  

Effects of AduoLa Fuzhenglin on changes of cytochrome c oxidase in rats′hippocampus after microwave exposure

作　　者：熊璐[1] 彭瑞云[1] 高亚兵[1] 王水明[1] 王丽峰[1] 董霁[1] 赵黎[1] 李杨[1] 左红艳[1] 徐新萍[1] 王晓民[1] 刘曙晨[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2011年第5期333-337,共5页Military Medical Sciences

基　　金：国家科技重大专项(2008ZXJ09004-019)

摘　　要：目的探讨中药复方制剂(安多霖)对微波辐射后大鼠海马线粒体呼吸链细胞色素C氧化酶(cytochromec oxidase,COX)变化的影响。方法 100只Wistar雄性大鼠随机分为3个对照组,即正常对照组、辐射预防对照组、辐射治疗对照组和2个安多霖组,即3 g/(kg.d)安多霖预防组及3 g/(kg.d)安多霖治疗组,每组20只。安多霖预防组大鼠于给药后第15天进行微波辐射;安多霖治疗组大鼠于辐射后当天开始连续给药14 d,均1次/d。采用30 mW/cm2微波辐射15 min,于辐射后6 h和14 d活杀动物取海马,通过比色法检测大鼠海马COX活性的变化;采用实时定量PCR和Western印迹检测大鼠海马COXⅠ、ⅣmRNA以及COXⅠ蛋白表达变化。结果辐射预防和治疗两对照组于停药后6 h COX活性、COXⅠ、ⅣmRNA,COXⅠ蛋白均降低(P<0.05或P<0.01);安多霖预防组较辐射预防对照组COX活性及基因表达有不同程度提高,与正常对照组无明显差异;安多霖治疗组与辐射治疗对照组相比明显提高(P<0.05),且基本恢复正常。结论给予3 g/(kg.d)安多霖对微波辐射致大鼠海马线粒体呼吸链COX表达降低有较明显改善作用,其治疗效果更为显著。Objective To investigate the effect of a herb compound AduoLa Fuzhenglin（ADL） on the gene cytochrome c oxidase（COX） change of the mitochondrial respiratory chain in rats′ hippocampus after microwave exposure.Methods One hundred Wistar male rats were exposed to microwave at the average power density of 30 mW/cm2for 15 min,3 g/（kg·d） ADL was administrated for 14 days before or after radiation.Rats were sacrificed at 6 h and 14 d after exposure.The activity of COX was determined by colorimetry;real time polymerase chain reaction was used to study the expression of COX Ⅰ and Ⅳ mRNA;the COX Ⅰ protein was detected by Western blot and image analysis.Results The level of COX activity,COX Ⅰ and Ⅳ mRNA and COX protein was decreased obviously in the radiated control group（P〈0.05 or P〈0.01） at 6 h and in the prophylaxis and treatment groups after drug withdrawal.The activity and gene expression of COX in the ADL prophylaxis group were higher than those in the radiated control group,and were not significant different from the normal control.Compared with the radiated control group,those in the ADL treatment group were significantly increased（P〈0.05）,and almost returned to normal.Conclusion Administration of 3 g/（kg·d） ADL could lessen the decrease in the COX expression level of the mitochondrial respiratory chain in rats′ hippocampus after microwave exposure,and with better effect on ADL treatment group.

关 键 词：安多霖 微波辐射 大鼠 海马 COX 

分 类 号：R285.5[医药卫生—中药学]