长期低剂量微波辐射对大鼠血清生化和心脏结构的影响研究  

Effects of long-term low doses of microwave radiation on biochemical indexes of serum and the myocardial histological structure in rats

作　　者：张静[1] 彭瑞云[1] 王水明[1] 高亚兵[1] 宋薇[1] 李翔[1] 董霁[1] 赵黎[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2011年第5期351-354,共4页Military Medical Sciences

基　　金：国家科技重大专项(2008ZXJ09004-019);全军医药卫生"十一五"科技攻关项目(08G145)

摘　　要：目的探讨长期低剂量微波辐射致大鼠血清生化、心脏功能及结构的影响。方法采用2.5、5和10 mW/cm2微波辐射160只二级雄性Wistar大鼠,5次/周×30 d,6 min/次。于辐射后1 d、14 d、60 d和180 d,采用全自动生化检测仪检测血清钾离子（K＋）、钠离子（Na＋）、天冬氨酸转氨酶（aspartate aminotransferase,AST）和谷氨酸转氨酶（alanine aminotransferase,ALT）含量;于辐射后6 h、7 d、14 d、30 d和60 d活杀大鼠取心脏,采用光镜和透射电镜观察心脏组织结构及超微结构的改变。结果 2.5、5和10 mW/cm2组大鼠血清Na＋含量分别于照射后1 d（P〈0.05）和14 d（P〈0.01）降低,60 d（P〈0.01）和180 d（P〈0.05）表达持续升高;AST和ALT含量分别于照射后14 d显著降低（P〈0.05）;180 d见恢复。2.5、5和10 mW/cm2微波辐射后7~30 d,大鼠心肌纤维排列紊乱,线粒体肿胀、空化,闰盘结构模糊,此病变随辐射剂量增加呈加重趋势。结论 2.5~10 mW/cm2微波长期辐射可致大鼠K＋、Na＋、AST及ALT含量异常及心脏组织结构与超微结构损伤。Objective To investigate the changes of the serum biochemical index and myocardial histological structure in rats after long-term low doses of microwave exposure.Methods One hundred and sixty male Wistar rats were continuously exposed to microwave of 2.5,5 and 10 mW/cm2 in a month,5 times a week and 6 minutes each time.The left ventricle blood was obtained at 1 d,14 d and 60 d after the rats were irradiated.The K＋,Na＋,AST and ALT level was analysed by Coulter automatic biochemical analyzer.All the experimental rats were sacrificed at 6 h,7 d,14 d,30 d and 60 d after exposure,then the histology and ultrastructure of the heart were observed by light and electron microscope.Results After 2.5,5 and 10 mW/cm2 microwave exposure,heart muscle fibers wavilness,mitochondrial swelling and cavitations,blurred intercalary dics were observed at 7 d-30 d.Conclusions A long-term of 2.5-10 mW/cm2 microwave exposure can cause the K＋,Na＋,AST and ALT turbulence,and injury to the histology and ultrastructure of the heart.

关 键 词：微波 大鼠 血清生化 心脏组织结构 

分 类 号：R144[医药卫生—公共卫生与预防医学]