微波辐射对猕猴血浆递质和血清代谢酶的影响  

Influence of microwave radiation on transmitters of blood plasma and metabolic enzymes in serum of macaque

作　　者：赵黎[1] 彭瑞云[1] 高亚兵[1] 王少霞[1] 王丽峰[1] 董霁[1] 王水明[1] 李杨[1] 徐新萍[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学》2011年第5期361-364,共4页Military Medical Sciences

基　　金：全军医药卫生"十一五"科技攻关项目(08G145)

摘　　要：目的探讨微波辐射对猕猴血浆氨基酸类递质和血清代谢酶的影响。方法实验用健康雄性猕猴15只,根据微波辐射平均功率的不同,随机分为5组,即对照组,2、5、7和11 mW/cm2组,每组3只,采用HPLC检测辐射后7 d和30 d猕猴血浆中氨基酸类递质含量的改变,采用全自动生化分析仪检测辐射后1,7,14,30和60 d猕猴血清代谢酶AST、LDH和CKMB的改变。结果微波辐射后7 d,5 mW/cm2组猕猴血浆中ASP和TAU显著增加（P〈0.05或P〈0.01）,11 mW/cm2组KYNA显著减少（P〈0.05）,TAU显著增加（P〈0.05）;辐射后30 d,11 mW/cm2组ASP显著减少（P〈0.01）,GLU和GLY显著增加（P〈0.05）。辐射后1 d,2和11 mW/cm2组猕猴血清中LDH显著减少（P〈0.05或P〈0.01）;辐射后7 d,5和11 mW/cm2组LDH显著增加（P〈0.05或P〈0.01）;辐射后14 d,5 mW/cm2组AST和LDH显著增加（P〈0.05或P〈0.01）,7和11 mW/cm2组CKMB显著降低（P〈0.05或P〈0.01）;辐射后60 d,11 mW/cm2组LDH显著减少（P〈0.05）。结论 2~11 mW/cm2微波辐射可造成猕猴血浆氨基酸类递质和血清代谢酶紊乱,且此紊乱程度与辐射剂量呈正相关。Objective To investigate the influence of microwave radiation on transmitters of blood plasma and metabolic enzymes in serum of macaque.Methods Fifteen male macaques were divided into 5 groups randomly according to the average power density,which were sham group,2,5,7 and 11 mW/cm2 groups.Amino acids transmitters in blood plasma were detected by HPLC at 7 d and 30 d after exposure,and aspartate transaminase（AST）,lactate dehydrogenase（LDH） and creatinkinase（CKMB） in serum were also analyzed by automatic biochemistry analyzer.Results At 7 d after microwave exposure,the content of aspartic acid（Asp） and taurine（Tau） in blood plasma increased（P〈0.05 or P〈0.01） in 5 mW/cm2 group,while the content of kynurenic acid（KYNA） decreased and Tau increased significantly in 11 mW/cm2 group（P〈0.05 or P〈0.01）.At 30 d after exposure,the content of Asp decreased and glutamic acid（Glu） and glycine（Gly） increased in 11 mW/cm2 group（P〈0.05 or P〈0.01）.The content of LDH in serum was decreased in 2 and 11 mW/cm2 groups at 1 d after exposure（P〈0.05 or P〈0.01）,but increased in 5 and 11 mW/cm2 groups at 7 d（P〈0.05 or P〈0.01）.At 14 d,the content of AST and LDH increased in 5 mW/cm2 group（P〈0.05 or P〈0.01） while the content of KYNA decreased in 7 and 11 mW/cm2 groups（P〈0.05 or P〈0.01）.At 60 d,the content of LDH decreased in 11 mW/cm2 group（P〈0.05）.Conclusion Microwave of 2,5 and 11 mW/cm2 can cause disturbance of amino acids transmitters in blood plasma and metabolic enzymes in serum,positively related to exposure doses.

关 键 词：微波 猕猴 外周血 递质 代谢酶 

分 类 号：Q691[生物学—生物物理学]