湖南现行家蚕品种资源对氟化物的抗性  

Resistance of the commercial silkworm races in Hunan province to fluoride

作　　者：艾均文 孟繁利 何行健 薛宏 黄仁志 赵玉文 

机构地区：[1]湖南省蚕桑科学研究所,湖南长沙410127

出　　处：《湖南农业大学学报（自然科学版）》2011年第3期280-282,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：现代农业(蚕桑)产业技术体系建设专项(nycytx-27-syz18);农业部公益性行业(农业)科研专项(nyhyzx07-020);湖南省科学技术厅项目(2010NK3050)

摘　　要：于4龄期分别采用150、300、600、1200、2400mg/kgNaF的2倍稀释液对湖南省现行家蚕22个品种资源进行添毒试验,通过机率值法和最小二乘法,分别得出NaF对各品种的毒力回归方程和对家蚕品种资源的半致死量(LC50)。结果表明:湖南省现行家蚕品种间的LC50差异较大,抗性强品种1514、秋白B、8535、夏芳B与秋丰B的LC50超过含抗氟主效基因的基础品种T6,而抗性弱品种云1、云2、皓月A、日3等的LC50低于1000mg/kg。In this study, the 22 different commercial silkworm races in Hunan province during 4th instar larvae were fed continuously on mulberry leaves immersed in serial two-fold dilutions of fluoride solutions with original concentration of 150, 300, 600, 1 200, 2 400 mg/kg respectively. The toxicological regression line（LD-P）,median lethal concentration （LCso）, 95 ~0 flducial limit of LC50, relation coefficient（r） and chi square test（X^2） were performed by a method according to the probit analysis and the least square. The bioassay result showed that there was distinct fluoride resistibility among these different commercial silkworm races in Hunan province. The commercial silkworm races ＂1514＂, ＂Qiubai B＂, ＂8535＂, ＂Xiafang B＂ and ＂Qiufeng B＂ were highly tolerant to fluoride, with LC50′S greater than that of silkworm strain ＂T6＂, which harbor the fluoride tolerance major gene, and the commercial silkworm races ＂Yun 1＂, ＂Yun 2＂, ＂Haoyue A＂ and ＂Ri 3＂ were highly sensitive to fluoride with LC50′s less than 1 000 mg/kg.

关 键 词：现行家蚕品种 抗氟性 半致死量 湖南 

分 类 号：S882.2[农业科学—特种经济动物饲养]