生防菌06-4对魔芋软腐病的防治及机理的初步研究  

Biocontrol effect of Lysobacter antibioticus 06-4 on soft rot pathogen of Amorphophallus konjac its mechanism

作　　者：张丽辉[1] 王永吉[1] 廖林[1] 姬广海[1] 

机构地区：[1]云南农业大学植物保护学院,云南昆明650201

出　　处：《湖南农业大学学报（自然科学版）》2011年第3期286-289,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：农业部农业行业科研专项(3-23);国家自然科学基金项目(30760139)

摘　　要：从拮抗作用、定殖特性、营养竞争方面研究溶杆菌属(Lysobacter)生防菌06-4对魔芋软腐病的生防机理。结果表明:生防菌06-4对不同致病力魔芋软腐病菌可产生拮抗作用;能在魔芋根部、根际土等生态位点定殖,定殖的量维持在102～104cfu/g,定殖时间长达32d,表现出良好的环境适应性和稳定性;与魔芋软腐病原菌具有46.88%的营养物质利用相似性,具有一定的营养竞争关系。2007年田间防效测试显示,在对照病情指数为41.20的情况下,生防菌06-4对魔芋软腐病的控制效果达到58.92%。Lysobaeter antibioticus strain 06-4 is a potential biocontrol agent against soft rot disease of Amorphophallus konjac root caused by Pectobacterium carotovora subsp, carotovora （Pcc）. Biocontrol mechanism of Lysobacter antibioticus strain 06-4 against Pcc was observed based on antagonism, colonization and nutrition competition. The results indicated that strain 06-4 showed antibacterial activity against Pcc strains with different virulence. It could colonize on the root and the rhizosphere of A.konjac and the organism could maintain 32 d with 10^2-10^4cfu/g. Nutritional requirement experiments showed that the similarity of sole-carbon-source utilization between Pcc and strain 06-4 was 46.88 %, suggesting a nutritional competition between the two microorganisms. Strain 06-4 also significantly reduced the bacterial soft rot index ofA. konjac by 58.92 % in field plot in 2007.

关 键 词：溶杆菌属 魔芋软腐病 定殖 营养竞争 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]