益生菌对异育银鲫生长和饲料消化率及RNA/DNA的影响  

Effect of probiotics on growth, digestibility and RNA/DNA of gibel carp

作　　者：陈勇[1,2] 周洪琪[2] 

机构地区：[1]南京晓庄学院生物化工与环境工程学院,江苏南京210017 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《湖南农业大学学报（自然科学版）》2011年第3期302-305,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：上海市科学技术委员会重点攻关项目(013212101);江苏省教育厅高校自然科学基金(08KJD180013);南京晓庄学院博士启动基金(2005NXY58)

摘　　要：在基础饲料中分别添加0(CK)、0.05%、0.20%和0.80%的益生菌制备试验饲料,饲养初重(78.31±2.15)g的异育银鲫64d,测定其特定生长率、饲料系数、蛋白质效率、成活率、异育银鲫肌肉营养成分及白肌RNA/DNA,并以Cr2O3为指示剂,采用间接法测定饲料消化率。结果表明:与对照相比,0.20%和0.80%益生菌添加组异育银鲫的特定增重率和特定增长率均显著提高(P<0.05),饲料系数显著降低(P<0.05),蛋白质效率显著提高(P<0.05),饲料的总消化率、饲料蛋白质消化率、脂肪消化率和异育银鲫白肌RNA/DNA均显著提高(P<0.05),除脂肪消化率和异育银鲫白肌RNA/DNA外,以上指标0.20%与0.80%益生菌添加组间的差异均不显著;添加0.80%的益生菌可以显著提高鱼体的蛋白质含量(P<0.05);考虑到成本因素,在饲料中添加0.20%的益生菌较为适宜。Gibel carp （initial weigh of （78.31＋2.15） g） were fed with basal diets and basal diets supplemented respectively with probiotics of 0.05%, 0.20% and 0.80% for 64 days. The survive ratio, the specific growth rate （SGR）, the feed coefficient, the protein efficiency, the muscle component and the RNA/DNA ratio in white muscle were measured and at the same time the digestibilities of the dietary nutriment were determined indirectly based on Cr203 to investigate influence of the probiotics on the digestion of the fish after 64-day feeding period. Results showed that the diets supplemented with 0.20% and 0.80% probiotics could significantly improve the SGR, protein efficiency （P〈0.05）, the gross digestibility and protein digestibility （P〈0.05）, and decrease the feed coefficient of gibel carp respectively （P〈0.05）. The supplement of 0.20% and 0.80% probiotics to the diets could also improve the fat digestibility and the RNA/DNA ratio in white muscle of fish significantly （P〈0.05）. Besides fat digestibility and RNA/DNA ratio in white muscle of fish, differences in the measured index between diets with 0.20% probiotics and those with 0.80% probiotics were insignificant （P〉0.05）. The diets supplemented with 0.80% probiotics could improve protein content in the fish muscle. The supplement of 0.20% probiotics in the diets is fitter considering cost.

关 键 词：异育银鲫 益生菌 生长 饲料消化率 RNA/DNA 

分 类 号：S963.73[农业科学—水产养殖]