应用响应面法优化超声波提取荆芥中总黄酮的工艺  

Optimization of Ultrasonic-assisted Extraction of Total Flavonoids from Schizonepeta Tenuifolia Briq by Response Surface Methodology

作　　者：刘奉强[1] 肖鉴谋[1] 刘太泽[1] 

机构地区：[1]南昌大学环境与化学工程学院,江西南昌330031

出　　处：《南昌大学学报（工科版）》2011年第2期149-155,共7页Journal of Nanchang University(Engineering & Technology)

基　　金：江西省教育厅科学技术研究基金资助项目(GJJ09037)

摘　　要：采用响应面法优化超声波提取荆芥中总黄酮的工艺。在单因素实验的基础上,选择乙醇体积分数、提取时间、提取温度和液料比作为实验因素,进行Box-Behnken中心组合实验设计,采用响应面法(RSM)评估了4个因素对总黄酮得率的影响。超声波法提取荆芥中总黄酮的最佳工艺条件如下:乙醇体积分数为56.2%,提取时间为45.3 min,超声功率为480 W,提取温度为60℃,液料比为27.9 mL/g。在最优的条件下,总黄酮得率为2.02%。Response surface methodology（RSM） was applied to optimize ultrasonic-assisted extraction process of total flavonoids from Schizonepeta Tenuifolia Briq.Based on single experiments,ethanol concentration,extraction time,extraction temperature and ratio of liquid to solid were selected for Box-Behnken central composite designing.RSM was employed to study the effect of these factors on the yield of total flavonoids.The results indicated that the optimal process conditions were ethanol concentration 56.2%,extraction time 45.3 min,ultrasonic power 480 W,extraction temperature 60 ℃ and ratio of liquid to solid 27.9 mL/g.Under these optimized conditions,the extraction yield of total flavonoids was up to 2.02%.

关 键 词：荆芥 总黄酮 超声波提取 响应面法 

分 类 号：TQ460.6[化学工程—制药化工]