基于聚硫堇/过氧化氢光电化学敏感界面的胆碱传感器  

Choline Biosensor Based on Poly(thionine)/H_2O_2 Photoelectrochemical Sensing Interface

作　　者：赵常志[1] 俞佳[1] 赵改爽[1] 焦奎[1] 

机构地区：[1]青岛科技大学化学与分子工程学院,青岛266042

出　　处：《分析化学》2011年第6期886-889,共4页Chinese Journal of Analytical Chemistry

基　　金：山东省自然科学基金(No.2009ZRB01393);电分析化学国家重点实验室开放基金资助

摘　　要：将胆碱氧化酶固定在具有光电活性的聚硫堇光透电极的表面,利用壳聚糖和戊二醛键合胆碱氧化酶制备了光致电化学胆碱传感器。此传感器基于同时具有光敏和电子受体功能的聚硫堇光电界面,能与胆碱氧化酶催化胆碱反应产生的电子供体(H2O2)发生光致电化学反应的原理,实现了对胆碱的检测。探讨了传感器的光致电化学响应机理、偏压、光强及电解质溶液pH对测定胆碱的影响。传感器对胆碱的测定范围为0.05～2.00 mmol/L;灵敏度为65.7 nA(mmol/L)-1;检出限为30μmol/L。此传感器的制备和检测不需要过氧化物酶及电子中介物,经济、简便且快速。A new choline biosensor based on the chitosan immobilized choline oxidase on poly（thionine） modified ITO electrode（PThE） was described.The photosensitive PThE was employed as an electron acceptor,and H2O2 produced by the catalytic reaction was employed as an electron donor.The photoelectrochemical responses of biosensor to H2O2 and choline were studied.The effects of bias voltage,light intensity and pH of electrolyte to photocurrent were investigated.The linear response of choline concentrations ranged from 0.05 to 2.00 mmol/L with a detection limit of 30.0 μmol/L and sensitivity of 65.7 nA/mmol/L.The applied feasibility of the biosensor was acknowledged through monitoring the phosphatidylcholine in lecithin samples.The prepared biosensor is economical,speedy,simple and convenient to detect the choline without hydrogen peroxidase.

关 键 词：胆碱传感器 光致电化学 聚硫堇 过氧化氢 胆碱 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]