HPLC-ELSD法检测鸟氨酸提取废液中的Ca^(2+)浓度  

Determination on Ca^(2+) Concentration in the Waste Liquid of L-Ornithine Extraction with HPLC-ELSD

作　　者：万红贵[1] 王汉领[1] 王燕[1] 将导航[1] 袁建锋[1] 李振江[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《安徽农业科学》2011年第15期8822-8823,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]为鸟氨酸发酵废液中Ca2+含量的测定提供参考。[方法]以七氟丁酸为柱中衍生试剂,采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法定量测定L-鸟氨酸发酵提取废液中Ca2+的含量。[结果]在反相C18色谱柱上,以浓度0.14%三氟乙酸(含0.08%七氟丁酸)水溶液-乙腈溶液(84∶16,V∶V)作为流动相,能有效地检测Ca2+含量,检出限为Ca2+浓度0.000 2～0.001 6 mol/L。其相应的浓度(Y)与峰面积(X)呈线性关系,线性方程为Y=7×10-10X-3×10-8,相关系数为1.000 0。Ca2+的平均回收率为99.83%～100.3%,相对标准偏差为0.61%～1.01%。[结论]该方法精密度高,准确度好,稳定性高,能简便、快速、准确地测定鸟氨酸发酵液中Ca2+的含量。[Objective]The purpose of the research was to provide reference for determining Ca2＋ content in the waste liquid of L-ornithine fermentation.[Method] With heptafluorobutyric acid as derivation reagent in column,the Ca2＋ content in the waste liquid of L-ornithine fermentation and extraction was determined quantitatively by high performance liquid chromatogram-evaporative light scattering detector.[Result] With 0.14% trifluoroacetic acid（0.08% heptafluorobutyric acid） aqueous solution-acetonitrile solution（84∶ 16,V∶ V） as mobile phase,the Ca2＋ content could be determined effectively using reverse phase C18 chromatographic column and its detection limit was 0.000 2~0.001 6 mol/L.The corresponding concn.（Y） and peak areas（X） showed linear relationship,their linear equation was Y=7×10-10X-3×10-8 and its correlation coefficient was 1.000 0.The average recovery of Ca2＋ was 99.83%-100.3% and its relative standard deviation was 0.61%~1.01%.[Conclusion] This method had high precision,accuracy and stability and the Ca2＋ content in the waste liquid of L-ornithine fermentation could be determined simply,rapidly and accurately by this method.

关 键 词：高效液相色谱-蒸发光散射检测器 七氟丁酸 CA2+ 

分 类 号：S188.4[农业科学—农业基础科学]