微量DNA高效提取方法  

Efficient Extracting Method of Minim DNA

作　　者：付少彬[1,2] 王海英[3] 孟庆峰 孙迪安[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]河北大学生命科学学院,河北保定071002 [3]山东师范大学,山东济南250014 [4]莱芜一中,山东莱芜271100

出　　处：《安徽农业科学》2011年第15期8868-8869,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探究一种高效的提取微量地衣体及地衣共生菌DNA的方法。[方法]在CTAB法的基础上,对溶液成分、比例及应用硅基质吸附柱对微量DNA提取进行优化。[结果]对于叶状、枝状和壳状地衣,采用该方法可得到质量较高的DNA。[结论]所用方法既节约样品,简便易行,费用低,稳定性好,效率高,而且不局限于新鲜材料。[Objective] A simple,efficient method for extraction of DNA for lichens and their mycobionts was studied.[Method] Based on CTAB method,the composition,the proportion of the solution and the application of the absorption column with silica substrate were optimized.[Result] The method was successfully applied for the species from different genus,including the foliose,fruticose and crustose efficiently.[Conclusion] The method was simple,convenient,inexpensive and not restricted to fresh material.

关 键 词：地衣 共生菌 微量DNA 提取方法 

分 类 号：S188[农业科学—农业基础科学]