反相高效液相色谱法同时测定香椿果中3种酚性化合物  

Simultaneous Determination of Three Phenolic Compounds in the Fruit of Toona sinensis(A. Juss.) Roem. by RP-HPLC

作　　者：李松林[1,2] 杨庆雄[1] 王聪聪[1] 田猛[1] 艾俊丽[1] 

机构地区：[1]贵州师范大学化学与材料科学学院,贵州贵阳550001 [2]贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵州贵阳550001

出　　处：《安徽农业科学》2011年第15期8908-8910,共3页Journal of Anhui Agricultural Sciences

基　　金：973计划前期研究专项项目(2007CB516813)

摘　　要：[目的]建立了同时测定香椿果提取物中没食子酸、没食子酸甲酯、1,2,3,4,6-五-O-没食子酰-β-D-葡萄糖3种酚性化合物含量的方法。[方法]采用Hypersil C18色谱柱(6.0 mm×150.0 mm,5μm),流动相为甲醇-0.1%甲酸,以1.0 ml/min流速为进行梯度洗脱,柱0.480、0.018～0.180和0.022～0.220μg之间线性关系良好(r≥0.999 8),平均加样回收率分别为101.6%、99.5%和99.1%,RSD值≤2.9%。[结论]该含量测定方法简便、准确、分离效果好,可用于香椿果中的酚性成分含量的测定。[Objective] To develop a high performance liquid chromatography（HPLC） method to simultaneously determine contents of three phenolic compounds including gallic acid,methyl gallate and 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose in the fruits of Toona sinensis（A.Juss.） Roem..[Method] The chromatographic separation was performed on a Hypersil C18 column（6.0 mm×150.0 mm,5 μm） with mobile phase of methanol and 0.1% formic acid in gradient elution whose flow rate was 1.0 ml/min.The temperature of sample manager was set at 30 ℃,and samples were detected at 280 nm and quantified by external standard method.[Result] The linear ranges of gallic acid,methyl gallate and 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose were respectively 0.048-0.480 μg,0.018-0.180 μg and 0.022-0.220 μg with correlation coefficient of more than or equal to 0.999 8.The average recovery of 3 phenolic compounds was respectively 101.6%,99.5% and 99.1% with RSD value of less than or equal to 2.9%.[Conclusion] The developed HPLC method was simple,sensitive and accurate with good repeatability,which was available for determining contents of phenolic compounds in the fruits of Toona sinensis（A.Juss.） Roem..

关 键 词：香椿果[Toona sinensis(A.Juss.)Roem] 反相高效液相色谱 没食子酸 没食子酸甲酯 1 2 3 4 6-五-O-没食子酰-β-D-葡萄糖 

分 类 号：S567[农业科学—中草药栽培]