肝癌细胞系Huh7中肿瘤干细胞分离及鉴定  

Isolation and Characterization of Cancer Stem Cells from a Human Hepatocellular Carcinoma Cell Lines Huh7

作　　者：梁晨[1] 单娟娟[1] 沈俊杰[1] 刘立[1] 钱程[1] 

机构地区：[1]浙江理工大学生命科学学院,杭州310018

出　　处：《浙江理工大学学报（自然科学版）》2011年第4期602-605,共4页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：国家自然科学基金面上项目(30872984);重庆市自然科学基金计划项目(CSTC09BB5024)

摘　　要：利用肿瘤干细胞具有自我更新能力的特点,首次采用悬浮培养技术从Huh7细胞系中得到肝癌(干)细胞球,并通过RT-PCR、流式细胞术和免疫荧光等方法检测它们的干细胞标记和分化标记。结果显示Huh7(干)细胞球的干性相关基因表达上调;分化相关基因表达下调;流式细胞仪检测结果表明88.82%的Huh7(干)细胞球表达EpCAM基因,与正常Huh7细胞相比表达上调。表明该方法具有富集肝癌干细胞的效果,为以后肝癌干细胞建立更高效的分离方法提供了一种新的技术方法。With the increasingly deep study on the mechanism of cancer,CSCs becomes a research focus.Several isolation methods are developed based on different phenotypes of CSCs.Spheroids derived from hepatocellular carcinoma cell line Huh7 cells are obtained by suspension culture based on the feature of CSC self-renewal.Stem cell markers and differentiation markers of the Huh7 spheroids are identified by RT-PCR method,FCM and IF.They are demonstrated to have elevated mRNA expressions of stem-cell-related genes and reduced levels of differentiation-related genes.Furthermore,88.82% of Huh7 spheroids are identified to express epithelial cell adhesion molecule（EpCAM）.In conclusion,these Huh7 spheroids may be the enrichment of cancer stem cells in Huh7 cells,providing a new approach to establish a more effective isolation method for liver cancer CSC in future.

关 键 词：肿瘤干细胞 肝癌(干)细胞球 生长因子 EPCAM 

分 类 号：Q786[生物学—分子生物学]