猪小肠粘膜下层作为组织工程肌腱支架的试验研究  

Pig Intestinal Submucosa as Tissue Engineering Scaffolds.Experimental Study of Tendon

作　　者：孙文才[1] 李玉成[1] 李博[1] 姜鑫[1] 李晓龙[1] 陈永春[1] 白玉江[1] 

机构地区：[1]齐齐哈尔医学院附属第三医院,黑龙江齐齐哈尔161000

出　　处：《中国伤残医学》2011年第6期17-19,共3页Chinese Journal of Trauma and Disability Medicine

基　　金：黑龙江省教育厅资助项目;项目编号:12511620

摘　　要：目的:制备小肠粘膜下层,用作肌腱替代物,观察其替代肌腱的过程和结果,检验体内组织工程理论的可行性。方法:取猪小肠的空肠部分,经化学处理后,将小肠粘膜下层卷曲成轴,取15只19天的罗曼鸡胚趾深屈肌腱,用酶消化方法体外分离培养肌腱细胞,并进行传代扩增。对各代肌腱细胞的活性与手术中进行比较。分别于术后第3、6、9周作为时间点,每个时间点处死8只动物切取左趾移植物,进行检测,所得数据进行处理和分析。结果:SIS替代肌腱的过程是代谢过程,经过16周,SIS为宿主肌腱完全替代,其组织结构和力学性能与正常肌腱极其接近。结论:体内组织工程理论至少在肌腱再生方面是可行的。复合细胞的SIS肌腱替代物优于单纯SIS替代肌腱的作用,SIS具有很好的生物相容性和力学强度,有可能成为肌腱组织工程的一种理想的支架材料。Objective： To observe its replacement hamstring tendon process and result, and inspection body tissue engineering theory is feasible of small intestinal submucosa,used for preparation of substitute. Methods： Taking pork small intestines, via the jejunum part after chemical treatment, small intestinal submucosa, curled up into a shaft of 19 days only take 15 Roman chicken embryos flexor tendon, toe deep enzyme digestion methods in vitro separation, and cultivating tendon cells nestin） amplification. Each generation of tendon cell activity compared with surgery. Respectively on postoperative 3, 6, and 9 weeks as time points, each time point executed only 8 animals take left toes cut, grafting, testing, income data processing and analysis. Results： SIS alternative hamstring process was metabolic processes, after 16 weeks, SIS for host tendon replace completely, its organizational structure and mechanical properties and was extremely close to normal tendons. Conclusion body tissue engineering theory at least in tendon regenerating is feasible.Conclusion： Body tissue engineering theory at least in tendon regenerating should be feasible. Composite cell SIS tendons should be better than pure SIS substitutes replace tendons role, SIS might have very good biocompatibility and mechanical strength, there may be a tendon tissue engineering scaffolds materials ideal.

关 键 词：组织工程 肌腱 支架 肌腱缺损 SIS 肌腱细胞 

分 类 号：R656.7[医药卫生—外科学]