共焦扫描成像研究  

Research on Confocal Scanning Imaging

作　　者：杨莉松[1] 王桂英[1] 王建岗[1] 徐至展[1] 

机构地区：[1]中国科学院上海光机所强光光学开放研究实验室,上海201800

出　　处：《中国激光》1999年第10期931-934,共4页Chinese Journal of Lasers

基　　金：国家自然科学重点基金!69637030;王宽成基金

摘　　要：在建立的一套反射式光纤共焦扫描成像系统上进行了标准板的扫描成像实验及反色、拼接等图像处理，得到了二维扫描像，并进行了二维共焦扫描像及离焦像的比较。实验结果表明，系统具有亚微米级横向分辨能力，并具有微米级纵向层析能力。Optical serial sectioning based on the depth-discriminating ability of a fiber optical confocal scanning microscope (FOCSM) can be combined with digital image processing to realize 3D microscopy. An instrument of FOCSM was designed for this purpose. In this paper, the 2D images of standard bars with some kinds of image processing in the FOCSM was shown. Furthermore, the in-focus image with the de-focus image has heen compared,and the 2D images for the further 3D reconstruction are analyzed.

关 键 词：反射式 光纤 共焦扫描成像 图像处理 

分 类 号：TN919.8[电子电信—通信与信息系统] T391.41[电子电信—信息与通信工程]