柴胡总皂苷提取工艺的优化  

Optimization of Extraction Technology for Saikosaponins from Bupleurum

作　　者：张国松[1] 封传华[2] 罗晓健[1,2] 宿士宁[2] 胡鹏翼[2] 王跃生[1,3] 

机构地区：[1]中药固体制剂制造技术国家工程研究中心,南昌330006 [2]江西中医学院,南昌330004 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2011年第12期17-20,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重大新药创制专项(2009ZX09103);江西省自然科学基金(201-GQY0048)

摘　　要：目的:考察柴胡总皂苷提取工艺。方法:以柴胡总皂苷量为指标,采用单因素和正交试验优选柴胡总皂苷的最佳提取工艺。结果:柴胡总皂苷最佳提取工艺为柴胡粉末(40目)加入8倍量80%乙醇,调节pH 8,80℃加热回流提取2次,每次60 min。结论:结果表明,该工艺简单、可靠、重复性好,适用于工业生产。Objective：To optimize the extraction technology for the saikosaponins from Bupleurum.Method：The extraction technology for the saikosaponins from Bupleurum was optimized by adopting a single factor experiment and an orthogonal experiment with the use of the extraction rate of the saikosaponins from Bupleurum as the assessment index.Result：The optimized extraction technology for the saikosaponins from Bupleurum was as follows：the powder of Bupleurum with a diameter less than 355 μm was subjected to reflux extraction for 2 times with 80% ethanol（regulate pH to 8） at 80 ℃,each extraction lasting 60 min.For each extraction,the solvent to solid ratio was 8.Conclusion：The extraction technology for the saikosaponins from Bupleurum is simple,reliable,repeatable and suitable for production in industry.

关 键 词：柴胡 柴胡总皂苷 提取工艺 正交试验 

分 类 号：R283.6[医药卫生—中药学]