星点设计-响应面法优选地桃花中总黄酮的提取工艺  

Application of CCD-RSM for Optimization of Total Flavonoids Extraction in Urena lobata

作　　者：谢臻[1] 陈勇[1] 曾海生[1] 韦韬[1] 卢森华[1] 

机构地区：[1]广西中医学院药学院,南宁530001

出　　处：《中国实验方剂学杂志》2011年第12期27-30,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西中医学院重点课题(ZD09011);广西研究生教育创新计划项目(2009106001007M)

摘　　要：目的:优选地桃花中总黄酮的提取工艺。方法:以乙醇体积分数、提取时间、溶剂量为考察因素,采用星点设计-响应面法,以地桃花中总黄酮的提取率为指标,优化地桃花的提取工艺条件。结果:确定最优提取工艺为55%乙醇,回流提取时间21 min,溶剂量17 mL。结论:优选得到的地桃花总黄酮提取工艺,方法简便。以此条件进行提取,可得到较高的总黄酮含量。Objective：To optimize the extracting process of total flavonoids for Urena lobata.Method：Independent variables were ethanol concentration,reflux time,and solvent,dependent variables were extracting rate of total flavonoids.Central composite design and response surface methodology were used to optimize the extracting process.Result：Analysis indicated that the optimum conditions were 55% ethanol,21 min,17 mL solvent for extractable conditions.Conclusion：The optimum process is simple and more convenient for extracting,by which higher total flavonoids content in Urena lobata L.can be obtaind.

关 键 词：地桃花 星点设计 响应面 总黄酮 

分 类 号：R283.6[医药卫生—中药学]