HPLC测定芩百清肺浓缩丸中次黄嘌呤  

Determination of Hypoxanthine in Qinbai Qingfei Concentrated Pills by HPLC

作　　者：王伟明[1] 霍金海[1] 孙国东[1] 

机构地区：[1]黑龙江省中医研究院,哈尔滨150036

出　　处：《中国实验方剂学杂志》2011年第12期70-71,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2010ZX09101-104)

摘　　要：目的:建立芩百清肺浓缩丸中次黄嘌呤的高效液相含量测定法。方法:采用HPLC-DAD法,色谱柱Diamonsil C18(4.6 mm×250 mm,5μm);流动相甲醇-水(2∶98),检测波长254 nm,流速1.0 mL·min-1,柱温30℃。结果:次黄嘌呤在1.94～31.04μg呈良好线性关系,r=0.999 9,平均回收率为99.14%,RSD 1.28%。结论:方法操作简便、灵敏度高、准确性和重复性好,可作为芩百清肺浓缩丸的质量控制方法。Objective：To establish the HPLC-DAD method for determination of the Hypoxanthine in Qinbaiqingfeinongsuo pills.Method：The separation was performed in a Diamonsil C18（4.6 mm×250 mm,5 μm） column with a mobile phase of methanol-water（2∶98） in a flow rate of 1.0 mL·min-1 elution at 254 nm and the column temperature was 30 ℃.Result：The linear range of Hypoxanthine was 1.94-31.04 mg·L-1（r=0.999 9）.The average recovery was 99.14%,RSD 1.28%.Conclusion：This method is simple,reliable,accurate,which offers advantages of higher sensitivity and precision.It can be used for quality control of Qinbai qingfei nongsuo pills.

关 键 词：芩百清肺浓缩丸 次黄嘌呤 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]