五味子复方对模拟航渡及高强度运动大鼠下丘脑-垂体-肾上腺轴功能和代谢的影响  

Effects of Schisandra Chinensis Fructus Compound on Function of HypothalaMuspituitary-Adrenal Axis and Metabolism in Rats Underwent Experimental Navigation and Strenuous Exercise

作　　者：黄慧[1] 孙玲君[1] 顾萍[1] 王坚[1] 

机构地区：[1]南京大学医学院临床学院南京军区南京总医院内分泌科,南京210002

出　　处：《中国实验方剂学杂志》2011年第12期136-140,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：南京军区"十一五"面上课题(06MA132)

摘　　要：目的:探讨五味子复方对模拟航渡及大强度运动大鼠下丘脑-垂体-肾上腺轴功能和代谢的影响。方法:选取31只大鼠,随机分成安静组(A组),应激组(B组)和五味子复方组(C组),大鼠给予五味子复方煎液10 mL.kg-1,2次/d ig,剂量为20 g·kg-1,共1周,B组和C组给予模拟渡航及高强度运动,比较3组大鼠血糖(blood glucose)、甘油三酯(triglyceride,TG)、胆固醇(cholesterol,CHO)及皮质醇(corticosterone,CORT)水平,并观察3组大鼠垂体促肾上腺皮质激素(adrenocorticotropichormone,ACTH)、神经肽Y(neuropeptide Y,NPY)阳性细胞数量和肾上腺皮质的超微结构。结果:①与A组相比,B组血糖水平显著增高(9.41±2.56)mmol.L-1,P<0.01,TG和CHO水平显著降低,TG(0.42±0.16)mmol.L-1,P<0.01;CHO(1.69±0.23)mmol.L-1,P<0.05;C组与B组相比,其血糖水平降低(6.45±2.25)mmol.L-1,P<0.05,TG水平升高(0.63±0.22)mmol.L-1,P<0.05,CHO无明显差异(1.83±0.19)mmol.L-1。②垂体ACTH和NPY阳性细胞免疫组化结果:C组ACTH阳性细胞和NPY阳性细胞数量较应激组增加,但两组都少于A组。③肾上腺电镜结果:与A组相比,B组肾上腺皮质细胞中分泌颗粒数目明显减少;C组肾上腺皮质细胞内分泌颗粒数目较B组明显增多。结论:五味子复方能降低应激大鼠血糖水平,维持物质代谢平衡,提高大鼠抗应激能力,保护垂体和肾上腺组织的结构功能。Objective：To investigate the influence of Schisandra compound on the function of the hypothalamus-pituitary-adrenal axis,the carbohydrate and blood-lipid metabolism in male rats underwent experimental navigation and strenuous exercise.Method：Thirty-one SD male rats were randomly allocated into three groups,quiet group（group A）,stress control group（group B） and Schisandra Chinensis Fructus compound administration（10 mL·kg-1,2/d,ig,one week） group（group C）.Both group B and C underwent wearing floating with psychological stress,then the corticosterone（CORT）,cholesterol（CHO）,triglyceride（TG）and blood glucose levels of the three groups were measured.At the same time,we excised the pituitary tissue to observe the number of the positive expression of ACTH and NPY cells by immunohistochemistry.Result：①In group B the plasma glucose level increased[（9.41±2.56） mmol·L-1,P0.01],TG and CHO levels decreased significantly compared to group A·L-1,P0.01];CHO[（1.69±0.23） mmol·L-1,P0.05].In group C,the plasma glucose level increased[（6.45±2.25） mmol·L-1,P0.05] and TG level decreased significantly compared to group B[（0.63±0.22） mmol·L-1,P0.05],but there＇s no obviouse difference in serum CHO level（1.83±0.19） mmol·L-1.②By immunohistochemistry,we found that in group C the number of the positive cells of ACTH and NPY in the pituitary tissue were increased compared to group B.However,the number of the positive cells of ACTH and NPY in both two groups were less than group A.③In group C,the number of the lipid granules increased obviously compared with group B,which is less than that in group A under electron microscope.Conclusion：Schisandra compound has the effects on adjusting serum levels of blood glucose and blood-fat,and can protect the structure of pituitary and adrenocortical cells.

关 键 词：五味子复方 模拟航渡 高强度运动 下丘脑-垂体-肾上腺轴 皮质醇 

分 类 号：R285.5[医药卫生—中药学]