软骨藻酸间接竞争ELISA检测方法的研究  

STUDY ON DETECTION METHOD OF INDIRECT-COMPETITIVE ELISA FOR DOMOIC ACID

作　　者：刘元嫄[1] 高利利[1] 程金平[1] 王茜[1] 王文华[1] 

机构地区：[1]上海交通大学环境科学与工程学院,上海200240

出　　处：《环境化学》2011年第6期1192-1196,共5页Environmental Chemistry

基　　金：上海市科委科技攻关项目(08DZ1206302);国家高新技术研究发展计划(863计划)(2007AA092001-15);海洋赤潮灾害立体监测技术与应用国家海洋局重点实验室开放研究基金(MATHAB200917)联合资助

摘　　要：为研究满足海产品日常分析的免疫检测法,采用自行制备的软骨藻酸多抗隆抗体,建立间接竞争ELISA检测方法,分别摸索了抗原、一抗、二抗的最佳使用浓度及温育温度与时间,并用该方法测定了贝类样品中的DA含量.结果表明,该方法最低检测限约为18 ng·mL-1,贝类样品测定的加标回收率在47.2%—94.2%之间.RSD在12.3%—17.0%之间.所购买3种的贝类样品中均无DA检出.An indirect-competitive enzyme-linked immunosorbent assay was established,using a self-prepared polyclonal antibody against domoic,for daily analysis of sea food.The working concentration of artificial antigen,antibody and HRP-IgG were measured.Best incubation condition,including temperature and time,was decided and several shellfish were detected.The results showed detection limit was about 18 ng·mL-1.Adding standard recovery ranged from 47.2% to 94.2% and RSD ranged from 12.3% to 17.0%.All results of three kinds of shellfish showed negative.

关 键 词：软骨藻酸 间接竞争ELISA 多克隆抗体 

分 类 号：X835[环境科学与工程—环境工程]