α-硫辛酸注射液对过氧化氢致PC12细胞损伤的保护作用  

Protective effect of α-lipoic acid injection on H_2O_2 injury in PC12 cell

作　　者：施红敏 韦晟[2] 

机构地区：[1]德清县人民医院药剂科,浙江德清313216 [2]南京市鼓楼医院药剂科,南京210008

出　　处：《中国临床药理学杂志》2011年第6期450-453,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究α-硫辛酸注射液(抗氧化剂)对H2O2致PC12细胞损伤的保护作用。方法 600μmol·L-1 H2O2与细胞共同孵育4 h,建立PC12细胞氧化应激损伤模型,用MTT法,测定细胞生长抑制率;培养介质中的LDH测定,用紫外分光光度法,用流式细胞术测定细胞凋亡率和线粒体膜电位。结果与模型组相比,3个剂量(10,1μmol·L-1)α-硫辛酸注射液能提高H2O2损伤的PC12细胞的存活率,减少LDH的漏出,降低细胞凋亡率,抑制线粒体膜电位的下降(P<0.05,P<0.01)。结论α-硫辛酸注射液对H2O2致PC12细胞损伤模型具有较好的保护作用。Objective To research the protective effects of α-lipoic acid injection on H2O2-induced cytotoxicity in PC12 cell line.Methods Oxidative stress injury of PC12 was produced by 600 μmol·L-1 H2O2 incubated with cells for 4 h.MTT method was used to test cell survival.Ultraviolet spectrophotometry was used to estimate LDH,and flow cytometry was used to determine cell apoptosis and mitochondria membrane potential.Results α-lipoic acid injection with concentrations of 10,1 μmol·L-1 could increase cell survival rate and mitochondria membrane potential,decrease supernatant LDH and cell apoptosis rate.Conclusion Oxidative stress injury in PC12 could be protected by α-lipoic acid injection.

关 键 词：α-硫辛酸注射液 PC12细胞 过氧化氢 

分 类 号：R977.9[医药卫生—药品] R587.2[医药卫生—药学]