低温人工脑脊液灌流对兔脊髓缺血再灌注时水通道蛋白-4表达的影响  

Effect of irrigation with hypothermic artificial cerebral spinal fluid on aquaporin-4 expression in spinal cord following spinal ischemia-reperfusion in rabbits

作　　者：唐昱英[1] 林严俊[2] 李吉满[3] 万俊峰[3] 黄蔚[1] 

机构地区：[1]四川大学华西第二医院麻醉科,成都市610041 [2]四川大学华西医院麻醉科 [3]四川省肿瘤医院病理科

出　　处：《中华麻醉学杂志》2011年第3期350-353,共4页Chinese Journal of Anesthesiology

基　　金：四川省科技厅科技支撑计划项目（2010SZ0185）

摘　　要：目的探讨低温人工脑脊液灌流对兔脊髓缺血再灌注时水通道蛋白．4（AQP-4）表达的影响。方法成年雄性新西兰大白兔54只，采用随机数字表法，将其随机分为3组（n=18）：假手术组（S组）、脊髓缺血再灌注组（I／R组）和低温人工脑脊液灌流组（FI组）。I／R组和FI组采用阻断肾下腹主动脉60min后开放的方法制备脊髓缺血再灌注模型。FI组阻断腹主动脉时以30ml／h的速率经L4．，间隙蛛网膜下腔输注25℃人工脑脊液，同时开放L7，8间隙蛛网膜下腔引流脑脊液。腹主动脉开放的同时停止人工脑脊液灌流。每组取6只动物，分别在再灌注4、24、48、72h时进行神经功能评分；每组分别于再灌注4、24、72h时取6只动物，取L5-8脊髓节段，计算脊髓组织含水量，并测定AQP-4表达水平。结果与S组比较，I／R组神经功能评分降低，脊髓组织含水量增加，AQP-4表达下调（P〈0．05）。与I／R组比较，FI组神经功能评分升高，脊髓组织含水量降低，AQP-4表达上调（P〈0．05）。结论低温人工脑脊液灌流可上调AQP-4表达，从而减轻兔脊髓缺血再灌注损伤。Objective To investigate the effect of irrigation with hypothermic artificial cerebral-spinal fluid （aCSF） on expression of aquaporin-4 （AQP-4） in the spinal cord following spinal ischemia-reperfusion （I/R） in rabbits. Methods Fifty-four adult male New Zealand white rabbits weighing 2.0-2.5 kg were randomly divided into 3 groups （n = 18 each）： group sham operation （S group）; group I/R and group irrigation with hypothermic aCSF （FI group）. Spinal I/R was induced by clamping the abdominal aorta below renal artery for 60 min. Hypot- hermic aCSF （25 ℃ ） was infused at L4,s interspace at a rate of 30 ml/h and drained from L7,8 interspace during spinal ischemia. Neurological function was evaluated at 4, 24, 48 and 72 h of reperfusion and scored （0 = no hind limb activity, 4 = hind limb function completely recovered） in 6 animals in each group. Six animals were sacrificed at 4, 24 and 72 h respectively in each group. The lumbar segment （ L5-8 ） was removed for measurement of water content and AQP-4 protein expression （by immuno-histochemistry）. Results Neurological function scores were significantly lower, water content was higher and AQP-4 expression smaller in group I/R than in group S. I/R-in- duced effects were significantly attenuated by irrigation of hypothermic aCSF. Conclusion Irrigation with hypothermic aCSF can ameliorate the spinal cord I/R injuries by up-regulation of AQP-4 expression.

关 键 词：低温 人工 脑脊髓液 再灌注损伤 脊髓 水通道蛋白质4 

分 类 号：R651.2[医药卫生—外科学]