肾综合征出血热乳鼠脑纯化疫苗(I型)糖蛋白及核蛋白在免疫保护中的作用  

The action of glycoprotein and nucleocapsid protein in immune protection which exist in hemorrhagic fever with renal syndrome vaccine

作　　者：王伟[1] 吴宏荣[1] 李娟[1] 王晶[1] 孙小慧[1] 王维新[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》1999年第4期27-29,共3页Progress In Microbiology and Immunology

摘　　要：为了阐明肾综合征出血热纯化疫苗（Ｉ型）中糖蛋白（ＧＰ）、核蛋白（ＮＰ）在免疫保护中的作用，采用脾细胞转输法，分别将ＧＰ、ＮＰ、ＧＰ＋ ＮＰ免疫小鼠后的脾细胞与未经免疫的小鼠正常脾细胞（３．５×１０５ 个）经腹腔转输给乳鼠，２ ｈ 后以１００ ＬＤ５０的汉滩（ＨＴＮ）病毒（ＬＲ１ 株）进行脑腔攻击。结果表明，其乳鼠的保护率分别达７７．０％ ，３８．５％ 和９２．３％ ，而未经免疫的小鼠正常脾细胞则不能提供保护（０％ ），揭示了ＧＰ＋ ＮＰ在抗ＨＴＮ 病毒感染中具有免疫协同作用。To explain the action of glycoprotein(GP) and nucleocapsid protein(NP) in immune protection which exist in hemorrhagic fever with renal syndrome(HFRS) vaccine.We have tested the protection ability of the mice spleen cells which were immuniged by GP,NP,GP and NP respectively.Intraperitonel(i·p)transfer of 3.5×10 6 immune cells respectively to infant mice(2～3 days old)2h after intracerebral(i·c)challenge with 100LD 50 of HTN virus(LR 1 strain)resulted in 77.0%,38.5%,92.3% protection respectively,in contrast,nonimmune spleen cells offered no pretection.The results suggested that GP and NP provide an immune cooperation in the resistrance of mice to HTN virus infection.

关 键 词：肾综合征出血热 纯化疫苗 糖蛋白 免疫保护 

分 类 号：R512.801[医药卫生—内科学]